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- W1844042730 abstract "Any reasonably modern fluorescence microscope can produce beautiful images of your cells and tissues without too much effort. But would you believe me if I told you that up to half of the images taken on our microscopes are essentially unquantifiable!? Lurking behind those colourful facades are: Lamp/ laser intensity fluctuations (as bad as 20% in a 3-hr session!)Non-uniform illumination across the field-of view (15–30% variations!)Uncorrected background and bleedthroughDark craters on camera surfacesPoor shutter control, leading to varying degrees of photobleachingDespite the fact that these (and other) issues are surprisingly widespread, users of these instruments are blissfully unaware of any problems and will likely go on to analyze the resulting images. I'll present several Case Studies that illustrate the extent of these problems, and then I'll suggest 7 specific steps that users can follow to ensure they get quantitative data from their microscope images." @default.
- W1844042730 created "2016-06-24" @default.
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- W1844042730 date "2013-05-01" @default.
- W1844042730 modified "2023-09-24" @default.
- W1844042730 title "You Call That Quantitative" @default.
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