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• W1844201680 abstract "Introduction: The p19 line of embryonal carcinoma cells develop into neurons, astroglia and fibroblasts after aggregation and exposure to retinoic acid (RA). Dehydroepiandrosterone (DHEA) is a neurosteroid, which can increase the proliferation of human neural stem cells (NSC) and positively regulate the number of neurons produced. This study was initiated to assess the effect of DHEA on neural progenitor cells derived from p19 embryonal carcinoma stem cells. Methods: p19 cells were suspended in DMEM containing 5% FBS in bacterial-grade Petri dishes in the presence of RA and DHEA at different concentrations for 6 days. Serum concentration was decreased to 3% on days 5 and 6. The aggregates were then collected and processed for flow cytometry, immunocytochemistry and RT-PCR analyses. Cells were trypsinized for dispersion and were placed in to poly-L-lysine (10 8g/ml) coated tissue culture dishes without RA and DHEA for 4 days. Differentiated cells were then evaluated by phase contrast microscopy. Results: Flow cytometry analyses of Nestin and Brdu/Nestin showed percent Nestin positive and proliferating Nestin positive cells in different groups including DHEA and RA groups. Brdu/Nestin immunochemistry confirmed proliferation of Nestin positive cells and also RT-PCR analysis showed the expression of proneural markers and estrogen receptor genes. Result showed that RA + DHEA (1µM) significantly increased the number of Nestin positive and newly formed Nestin positive cells compared to other groups. Conclusion: These results showed that DHEA accompanied by RA significantly increased the number of Nestin positive and newly formed Nestin positive cells derived from p19 embryonal carcinoma cells but in comparison to RA could not induce neural progenitor cells." @default.
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• W1844201680 date "2008-11-10" @default.
• W1844201680 modified "2023-09-25" @default.
• W1844201680 title "DEHYDROEPIANDROSTERONE INCREASES THE PROLIFERATION OF NEURAL PROGENITOR CELLS DERIVED FROM P19 EMBRYONAL CARCINOMA STEM CELLS" @default.
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