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- W1844229196 abstract "The important antigen site of S and N genes of the SC-H strain of TGVE was amplified by RT-PCR and cloned into pMD-19T vector,the recombinant was named 19TS and 19T-N,which was identified by restriction enzyme and sequenced.Then the S and N genes were cut from the recombinant plasmid 19T-S and 19T-N,further inserted into the expression vector pVAX1 got S/N chimeric eukaryotic expression plasmid pVAX-S-N.After identified by restriction enzyme and PCR,the recombinant plasmids were tranfected into COS7 cells,the expressions of recombinant plasmids were confirmed by indirect immunofluorscence assay.The results showed that the eukaryotic expression plasmids were constructed successfully and the transfected cells displayed specific immunofluorscence.The successful construction of the pVAX-S-N provides foundation for advance research of the TGEV DNA vaccine." @default.
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- W1844229196 date "2009-01-01" @default.
- W1844229196 modified "2023-09-24" @default.
- W1844229196 title "Construction and expression of TGEV chimeric plasmid pVAX-S-N." @default.
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