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- W184424002 abstract "Immunohistochemistry has rapidly acquired an important position in present-day pathology laboratories. The expanding selection of monoclonal and poly-clonal antibodies with well defined specificities has greatly increased the potentially obtainable information from routine biopsy specimens. Basically, the only limiting factors for the demonstration of the presence of any biologically significant, antigenic molecules are the specificity of the antibody on one hand and, on the other, the tolerance of the epitope(s) to the various methods required for the handling of the specimens. Appropriate characterization of the antibodies used, whether polyclonal or monoclonal is imperative. They should be thoroughly analysed for lack of cross reactions and in electrophoretic blots the antibodies should react only with the corresponding antigen. On the target side, overall the best results are obtained with frozen sections, fixed for instance in acetone. Alcohol or Bouin fixation followed by paraffin embedding also gives generally good results, although the long-term preservation of the material is less good. Formalin fixed and paraffin embedded material can be successfully applied in many instances although the sensitivity of detecting certain antigens including intermediate filaments can be markedly decreased. Enzyme pre-treatment of such sections can often markedly improve the results probably by degrading the cross-links that may mask the antigenic sites of many molecules. Inclusion of proper controls into each diagnostic run is of course mandatory. The technical aspects have been considered in several excellent reviews (e.g. Taylor 1978; Lewis et al. 1983)." @default.
- W184424002 created "2016-06-24" @default.
- W184424002 creator A5071034988 @default.
- W184424002 date "1989-01-01" @default.
- W184424002 modified "2023-10-15" @default.
- W184424002 title "Advances in Immunohistochemistry of Ovarian Tumours" @default.
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- W184424002 cites W1536695820 @default.
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- W184424002 cites W1968505371 @default.
- W184424002 cites W1969007097 @default.
- W184424002 cites W1973952885 @default.
- W184424002 cites W1975780240 @default.
- W184424002 cites W1976048583 @default.
- W184424002 cites W1977305271 @default.
- W184424002 cites W1978246084 @default.
- W184424002 cites W1994330012 @default.
- W184424002 cites W1994745314 @default.
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- W184424002 cites W2003397326 @default.
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- W184424002 cites W2016417001 @default.
- W184424002 cites W2019722811 @default.
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- W184424002 doi "https://doi.org/10.1007/978-3-642-74011-4_7" @default.
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