FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1844301679> ?p ?o ?g. }

• W1844301679 endingPage "260" @default.
• W1844301679 startingPage "251" @default.
• W1844301679 abstract "BackgroundCalcific aortic valve disease (CAVD) is the most common heart valve disease in the Western world. We previously proposed that valvular endothelial cells (VECs) replenish injured adult valve leaflets via endothelial-to-mesenchymal transformation (EndMT); however, whether EndMT contributes to valvular calcification is unknown. We hypothesized that aortic VECs undergo osteogenic differentiation via an EndMT process that can be inhibited by valvular interstitial cells (VICs).Approach and resultsVEC clones underwent TGF-β1-mediated EndMT, shown by significantly increased mRNA expression of the EndMT markers α-SMA (5.3 ± 1.2), MMP-2 (13.5 ± 0.6) and Slug (12 ± 2.1) (p < 0.05), (compared to unstimulated controls). To study the effects of VIC on VEC EndMT, clonal populations of VICs were derived from the same valve leaflets, placed in co-culture with VECs, and grown in control/TGF-β1 supplemented media. In the presence of VICs, EndMT was inhibited, shown by decreased mRNA expression of α-SMA (0.1 ± 0.5), MMP-2 (0.1 ± 0.1), and Slug (0.2 ± 0.2) (p < 0.05). When cultured in osteogenic media, VECs demonstrated osteogenic changes confirmed by increase in mRNA expression of osteocalcin (8.6 ± 1.3), osteopontin (3.7 ± 0.3), and Runx2 (5.5 ± 1.5). The VIC presence inhibited VEC osteogenesis, demonstrated by decreased expression of osteocalcin (0.4 ± 0.1) and osteopontin (0.2 ± 0.1) (p < 0.05). Time course analysis suggested that EndMT precedes osteogenesis, shown by an initial increase of α-SMA and MMP-2 (day 7), followed by an increase of osteopontin and osteocalcin (day 14).ConclusionsThe data indicate that EndMT may precede VEC osteogenesis. This study shows that VICs inhibit VEC EndMT and osteogenesis, indicating the importance of VEC–VIC interactions in valve homeostasis." @default.
• W1844301679 created "2016-06-24" @default.
• W1844301679 creator A5006481103 @default.
• W1844301679 creator A5015785113 @default.
• W1844301679 creator A5022559566 @default.
• W1844301679 creator A5023064529 @default.
• W1844301679 creator A5058467567 @default.
• W1844301679 creator A5064521237 @default.
• W1844301679 creator A5065204696 @default.
• W1844301679 creator A5066532151 @default.
• W1844301679 creator A5073253770 @default.
• W1844301679 date "2015-09-01" @default.
• W1844301679 modified "2023-10-14" @default.
• W1844301679 title "Valvular interstitial cells suppress calcification of valvular endothelial cells" @default.
• W1844301679 cites W1507413178 @default.
• W1844301679 cites W1807444114 @default.
• W1844301679 cites W1824991881 @default.
• W1844301679 cites W1829900333 @default.
• W1844301679 cites W19217443 @default.
• W1844301679 cites W1967483573 @default.
• W1844301679 cites W1985382259 @default.
• W1844301679 cites W1987650867 @default.
• W1844301679 cites W2039182800 @default.
• W1844301679 cites W2040321633 @default.
• W1844301679 cites W2043635105 @default.
• W1844301679 cites W2047368989 @default.
• W1844301679 cites W2055653999 @default.
• W1844301679 cites W2064092148 @default.
• W1844301679 cites W2073982480 @default.
• W1844301679 cites W2079284897 @default.
• W1844301679 cites W2085830973 @default.
• W1844301679 cites W2091867261 @default.
• W1844301679 cites W2094391275 @default.
• W1844301679 cites W2096132697 @default.
• W1844301679 cites W2098019840 @default.
• W1844301679 cites W2102103202 @default.
• W1844301679 cites W2103757269 @default.
• W1844301679 cites W2104974378 @default.
• W1844301679 cites W2125767846 @default.
• W1844301679 cites W2127317998 @default.
• W1844301679 cites W2131655610 @default.
• W1844301679 cites W2131672223 @default.
• W1844301679 cites W2138229905 @default.
• W1844301679 cites W2143532796 @default.
• W1844301679 cites W2149932164 @default.
• W1844301679 cites W2150376368 @default.
• W1844301679 cites W2151796223 @default.
• W1844301679 cites W2152442991 @default.
• W1844301679 cites W2162452658 @default.
• W1844301679 cites W2163408805 @default.
• W1844301679 cites W2170326026 @default.
• W1844301679 cites W2171108436 @default.
• W1844301679 cites W2171894777 @default.
• W1844301679 cites W4248259718 @default.
• W1844301679 doi "https://doi.org/10.1016/j.atherosclerosis.2015.07.008" @default.
• W1844301679 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4546848" @default.
• W1844301679 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26232165" @default.
• W1844301679 hasPublicationYear "2015" @default.
• W1844301679 type Work @default.
• W1844301679 sameAs 1844301679 @default.
• W1844301679 citedByCount "127" @default.
• W1844301679 countsByYear W18443016792015 @default.
• W1844301679 countsByYear W18443016792016 @default.
• W1844301679 countsByYear W18443016792017 @default.
• W1844301679 countsByYear W18443016792018 @default.
• W1844301679 countsByYear W18443016792019 @default.
• W1844301679 countsByYear W18443016792020 @default.
• W1844301679 countsByYear W18443016792021 @default.
• W1844301679 countsByYear W18443016792022 @default.
• W1844301679 countsByYear W18443016792023 @default.
• W1844301679 crossrefType "journal-article" @default.
• W1844301679 hasAuthorship W1844301679A5006481103 @default.
• W1844301679 hasAuthorship W1844301679A5015785113 @default.
• W1844301679 hasAuthorship W1844301679A5022559566 @default.
• W1844301679 hasAuthorship W1844301679A5023064529 @default.
• W1844301679 hasAuthorship W1844301679A5058467567 @default.
• W1844301679 hasAuthorship W1844301679A5064521237 @default.
• W1844301679 hasAuthorship W1844301679A5065204696 @default.
• W1844301679 hasAuthorship W1844301679A5066532151 @default.
• W1844301679 hasAuthorship W1844301679A5073253770 @default.
• W1844301679 hasBestOaLocation W18443016791 @default.
• W1844301679 hasConcept C104317684 @default.
• W1844301679 hasConcept C105580179 @default.
• W1844301679 hasConcept C126322002 @default.
• W1844301679 hasConcept C131075544 @default.
• W1844301679 hasConcept C134018914 @default.
• W1844301679 hasConcept C142724271 @default.
• W1844301679 hasConcept C150194340 @default.
• W1844301679 hasConcept C160160445 @default.
• W1844301679 hasConcept C181199279 @default.
• W1844301679 hasConcept C185592680 @default.
• W1844301679 hasConcept C198826908 @default.
• W1844301679 hasConcept C2776363554 @default.
• W1844301679 hasConcept C2777534427 @default.
• W1844301679 hasConcept C2780309369 @default.
• W1844301679 hasConcept C2780714102 @default.
• W1844301679 hasConcept C2780804394 @default.
• W1844301679 hasConcept C55493867 @default.

• next