Matches in SemOpenAlex for { <https://semopenalex.org/work/W1844476724> ?p ?o ?g. }
- W1844476724 abstract "In this current study, we are investigating the influence of 6-bromo-indirubin-3’oxime (6BIO) and 7-bromo-indirubin-3’oxime (7BIO) on induction of apoptosis, cell proliferation, and anti-migratory effects in the well characterized pancreatic carcinoma L3.6pl and breast carcinoma Skbr3 tumor cell lines and characterize underlying mechanisms. 6BIO and 7BIO at doses of 10 µM were shown to significantly reduce the proliferation and viability as well as induce apoptosis in both cell lines. In addition, 6BIO, but not 7BIO, significantly reduced the migration of both cell lines, nearly halting them completely in the Skbr3 wound healing assay at sub-apoptotic doses (3 µM). Chemotaxis was dramatically disrupted and tumor cells significantly lost their ability to invade through membranes or MatrigelTM layers in response to chemoattractants. An increase of the phosphorylation site S785 of beta1 integrin is seen upon 6BIO which has been linked to decreased motility of carcinoma cells. Additionally, adhesion of Skbr3 tumor cells to fibronectin was reduced by 6BIO stimulation. The effects of 6BIO can be attributed to its reduction of the T308 phosphorylation site of Akt, most likely through its direct inhibition of PDK1, ultimately causing long term alterations to the actin cytoskeleton. Erk, FAK and Rac1 levels are unaffected, but cycling of these signaling molecules appears to be disrupted upon treatment. Finally 6BIO reduced the metabolic capabilities of Skbr3 spheroids at low doses, caused the dissolution of spheroid structures at higher doses and significantly blocked the migration of Skbr3 spheroids. Taken together, the results of this study strongly suggest that the indirubin derivative 6BIO operates by inhibiting different mechanisms in human tumor cells to exert their potent anti-tumor efficacy." @default.
- W1844476724 created "2016-06-24" @default.
- W1844476724 creator A5047836189 @default.
- W1844476724 date "2010-07-13" @default.
- W1844476724 modified "2023-09-22" @default.
- W1844476724 title "Anticancer effects and antimetastatic mechanisms of novel indirubin derivatives" @default.
- W1844476724 cites W12851679 @default.
- W1844476724 cites W133150085 @default.
- W1844476724 cites W1545201063 @default.
- W1844476724 cites W1576773659 @default.
- W1844476724 cites W1606209721 @default.
- W1844476724 cites W1806339493 @default.
- W1844476724 cites W1910650341 @default.
- W1844476724 cites W1964378600 @default.
- W1844476724 cites W1967934035 @default.
- W1844476724 cites W1968952749 @default.
- W1844476724 cites W1969063564 @default.
- W1844476724 cites W1971763871 @default.
- W1844476724 cites W1973800056 @default.
- W1844476724 cites W1976378285 @default.
- W1844476724 cites W1979129905 @default.
- W1844476724 cites W1981684516 @default.
- W1844476724 cites W1983473247 @default.
- W1844476724 cites W1984168023 @default.
- W1844476724 cites W1988006764 @default.
- W1844476724 cites W1989060543 @default.
- W1844476724 cites W1993860023 @default.
- W1844476724 cites W1998038367 @default.
- W1844476724 cites W2001271989 @default.
- W1844476724 cites W2002963357 @default.
- W1844476724 cites W2003529692 @default.
- W1844476724 cites W2009644972 @default.
- W1844476724 cites W2010618491 @default.
- W1844476724 cites W2021683914 @default.
- W1844476724 cites W2027698698 @default.
- W1844476724 cites W2027802778 @default.
- W1844476724 cites W2028497798 @default.
- W1844476724 cites W2029525135 @default.
- W1844476724 cites W2034227299 @default.
- W1844476724 cites W2034833395 @default.
- W1844476724 cites W2037120935 @default.
- W1844476724 cites W2039193709 @default.
- W1844476724 cites W2040410288 @default.
- W1844476724 cites W2041163647 @default.
- W1844476724 cites W2042151520 @default.
- W1844476724 cites W2042683127 @default.
- W1844476724 cites W2044273547 @default.
- W1844476724 cites W2045604110 @default.
- W1844476724 cites W2046847991 @default.
- W1844476724 cites W2050108686 @default.
- W1844476724 cites W2050865455 @default.
- W1844476724 cites W2052201526 @default.
- W1844476724 cites W2052276044 @default.
- W1844476724 cites W2056657105 @default.
- W1844476724 cites W2060771546 @default.
- W1844476724 cites W2061180610 @default.
- W1844476724 cites W2063220206 @default.
- W1844476724 cites W2064073012 @default.
- W1844476724 cites W2069924164 @default.
- W1844476724 cites W2071637341 @default.
- W1844476724 cites W2073891485 @default.
- W1844476724 cites W2074787208 @default.
- W1844476724 cites W2076042473 @default.
- W1844476724 cites W2076830904 @default.
- W1844476724 cites W2082822758 @default.
- W1844476724 cites W2084366893 @default.
- W1844476724 cites W2084867085 @default.
- W1844476724 cites W2084951981 @default.
- W1844476724 cites W2085593164 @default.
- W1844476724 cites W2087861588 @default.
- W1844476724 cites W2087870568 @default.
- W1844476724 cites W2087963453 @default.
- W1844476724 cites W2089426706 @default.
- W1844476724 cites W2093921289 @default.
- W1844476724 cites W2095795258 @default.
- W1844476724 cites W2100837269 @default.
- W1844476724 cites W2104150310 @default.
- W1844476724 cites W2104373322 @default.
- W1844476724 cites W2107487363 @default.
- W1844476724 cites W2110228428 @default.
- W1844476724 cites W2116369738 @default.
- W1844476724 cites W2116504572 @default.
- W1844476724 cites W2118299978 @default.
- W1844476724 cites W2121394053 @default.
- W1844476724 cites W2122572948 @default.
- W1844476724 cites W2123409005 @default.
- W1844476724 cites W2126176773 @default.
- W1844476724 cites W2126496496 @default.
- W1844476724 cites W2127481599 @default.
- W1844476724 cites W2128635872 @default.
- W1844476724 cites W2131534333 @default.
- W1844476724 cites W2141617119 @default.
- W1844476724 cites W2145009320 @default.
- W1844476724 cites W2146157811 @default.
- W1844476724 cites W2146894821 @default.
- W1844476724 cites W2147000598 @default.
- W1844476724 cites W2147680105 @default.
- W1844476724 cites W2150026213 @default.
- W1844476724 cites W2153956427 @default.
- W1844476724 cites W2159990074 @default.