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- W1844927061 abstract "Research Article1 March 1984free access Integrity of the pericellular fibronectin matrix of fibroblasts is independent of sulfated glycosaminoglycans. K. Hedman K. Hedman Search for more papers by this author T. Vartio T. Vartio Search for more papers by this author S. Johansson S. Johansson Search for more papers by this author L. Kjellén L. Kjellén Search for more papers by this author M. Höök M. Höök Search for more papers by this author A. Linker A. Linker Search for more papers by this author E.M. Salonen E.M. Salonen Search for more papers by this author A. Vaheri A. Vaheri Search for more papers by this author K. Hedman K. Hedman Search for more papers by this author T. Vartio T. Vartio Search for more papers by this author S. Johansson S. Johansson Search for more papers by this author L. Kjellén L. Kjellén Search for more papers by this author M. Höök M. Höök Search for more papers by this author A. Linker A. Linker Search for more papers by this author E.M. Salonen E.M. Salonen Search for more papers by this author A. Vaheri A. Vaheri Search for more papers by this author Author Information K. Hedman, T. Vartio, S. Johansson, L. Kjellén, M. Höök, A. Linker, E.M. Salonen and A. Vaheri The EMBO Journal (1984)3:581-584https://doi.org/10.1002/j.1460-2075.1984.tb01851.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The pericellular matrix fibers of cultured human fibroblasts contain fibronectin, other glycoproteins, and heparan and chondroitin sulfate proteoglycans. In the present study, cell-free pericellular matrices were isolated from metabolically labeled fibroblast cultures. The isolated matrices were digested with heparinase from Flavobacterium heparinum, and then analyzed for sulfated glycosaminoglycans (GAGs). Nitrous acid degradation was used to distinguish the N-sulfated GAGs (heparan sulfate) from chondroitin sulfate. Fibronectin and the other major matrix polypeptides were studied using gel electrophoresis, enzyme immunoassay and immunofluorescence. Upon heparinase digestion, greater than 95% of sulfated GAGs were degraded in the matrix without detectable release of fibronectin or other matrix polypeptides or alteration of the fibrillar matrix structure. We conclude that in fibroblast cultures the integrity of the fibrillar matrix is independent of sulfated GAGs. Together with earlier observations, this suggests that filamentous polymerization of fibronectin forms the backbone of early connective tissue matrix. Previous ArticleNext Article Volume 3Issue 31 March 1984In this issue RelatedDetailsLoading ..." @default.
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- W1844927061 title "Integrity of the pericellular fibronectin matrix of fibroblasts is independent of sulfated glycosaminoglycans." @default.
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