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• W1844946935 abstract "This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age.Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The zona pellucida was thinned to more than 70% of its initial thickness by making two holes of 15-20 µm.In the young mice, LAZT resulted in a significant increase in early hatching and hatching rates compared to the control group (p<0.05). However, in the old mice, LAZT significantly increased blastocyst formation as well as early hatching and hatching compared to the controls (p<0.05). These effects were more remarkable in LAZT4 than in LAZT1 and in aged mice than in young ones.These results show that multi-point LAZT leads to a significant improvement of blastocyst formation and hatching in mice compared to controls." @default.
• W1844946935 created "2016-06-24" @default.
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• W1844946935 date "2015-01-01" @default.
• W1844946935 modified "2023-10-05" @default.
• W1844946935 title "Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice" @default.
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• W1844946935 doi "https://doi.org/10.5653/cerm.2015.42.2.51" @default.
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