Matches in SemOpenAlex for { <https://semopenalex.org/work/W1845212532> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W1845212532 endingPage "854" @default.
- W1845212532 startingPage "847" @default.
- W1845212532 abstract "Our renin IRMA overestimated renin in plasmas with high prorenin-to-renin ratios. We suspected that the overestimation of renin was caused less by cross-reactivity of the renin-specific antibody with prorenin than by a conformational change of prorenin into an enzymatically active form during the assay.Because the inactive form of prorenin converts slowly into an active form at low temperature, we raised the assay temperature from 22 degrees C to 37 degrees C, simultaneously shortening the incubation time from 24 to 6 h. The former IRMA was performed in <1 working day with these modifications.The comeasurement of prorenin as renin was eliminated. Reagents were stable at 37 degrees C, and the new and old IRMAs were comparable in terms of precision and accuracy. The functional lower limit of the assay (4 mU/L) was below the lower reference limit (9 mU/L). The modified IRMA agreed closely with the activities measured with an enzyme-kinetic assay. Results were not influenced by the plasma concentration of angiotensinogen. At normal angiotensinogen concentrations, the IRMA closely correlated with the classical enzyme-kinetic assay of plasma renin activity.The modified IRMA, performed at 37 degrees C, avoids interference by prorenin while retaining the desirable analytical characteristics of the older IRMA and requiring less time." @default.
- W1845212532 created "2016-06-24" @default.
- W1845212532 creator A5021542514 @default.
- W1845212532 creator A5065918773 @default.
- W1845212532 creator A5077608155 @default.
- W1845212532 date "1999-06-01" @default.
- W1845212532 modified "2023-09-27" @default.
- W1845212532 title "Improved Immunoradiometric Assay for Plasma Renin" @default.
- W1845212532 cites W1541747676 @default.
- W1845212532 cites W1594509828 @default.
- W1845212532 cites W1822076357 @default.
- W1845212532 cites W1827617621 @default.
- W1845212532 cites W2012866751 @default.
- W1845212532 cites W2035943436 @default.
- W1845212532 cites W2050325550 @default.
- W1845212532 cites W2058179499 @default.
- W1845212532 cites W2074588444 @default.
- W1845212532 cites W2094857198 @default.
- W1845212532 cites W2147355520 @default.
- W1845212532 cites W2160826984 @default.
- W1845212532 cites W2162403411 @default.
- W1845212532 cites W2166377713 @default.
- W1845212532 cites W2166750909 @default.
- W1845212532 cites W2266505793 @default.
- W1845212532 cites W4211064734 @default.
- W1845212532 cites W1925446680 @default.
- W1845212532 doi "https://doi.org/10.1093/clinchem/45.6.847" @default.
- W1845212532 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10351994" @default.
- W1845212532 hasPublicationYear "1999" @default.
- W1845212532 type Work @default.
- W1845212532 sameAs 1845212532 @default.
- W1845212532 citedByCount "48" @default.
- W1845212532 countsByYear W18452125322012 @default.
- W1845212532 countsByYear W18452125322013 @default.
- W1845212532 countsByYear W18452125322015 @default.
- W1845212532 countsByYear W18452125322016 @default.
- W1845212532 countsByYear W18452125322017 @default.
- W1845212532 countsByYear W18452125322018 @default.
- W1845212532 countsByYear W18452125322019 @default.
- W1845212532 countsByYear W18452125322023 @default.
- W1845212532 crossrefType "journal-article" @default.
- W1845212532 hasAuthorship W1845212532A5021542514 @default.
- W1845212532 hasAuthorship W1845212532A5065918773 @default.
- W1845212532 hasAuthorship W1845212532A5077608155 @default.
- W1845212532 hasBestOaLocation W18452125321 @default.
- W1845212532 hasConcept C119128265 @default.
- W1845212532 hasConcept C126322002 @default.
- W1845212532 hasConcept C134018914 @default.
- W1845212532 hasConcept C138976137 @default.
- W1845212532 hasConcept C176685330 @default.
- W1845212532 hasConcept C181199279 @default.
- W1845212532 hasConcept C185592680 @default.
- W1845212532 hasConcept C198710026 @default.
- W1845212532 hasConcept C25642318 @default.
- W1845212532 hasConcept C2778758028 @default.
- W1845212532 hasConcept C43617362 @default.
- W1845212532 hasConcept C55493867 @default.
- W1845212532 hasConcept C71924100 @default.
- W1845212532 hasConcept C84393581 @default.
- W1845212532 hasConcept C86803240 @default.
- W1845212532 hasConceptScore W1845212532C119128265 @default.
- W1845212532 hasConceptScore W1845212532C126322002 @default.
- W1845212532 hasConceptScore W1845212532C134018914 @default.
- W1845212532 hasConceptScore W1845212532C138976137 @default.
- W1845212532 hasConceptScore W1845212532C176685330 @default.
- W1845212532 hasConceptScore W1845212532C181199279 @default.
- W1845212532 hasConceptScore W1845212532C185592680 @default.
- W1845212532 hasConceptScore W1845212532C198710026 @default.
- W1845212532 hasConceptScore W1845212532C25642318 @default.
- W1845212532 hasConceptScore W1845212532C2778758028 @default.
- W1845212532 hasConceptScore W1845212532C43617362 @default.
- W1845212532 hasConceptScore W1845212532C55493867 @default.
- W1845212532 hasConceptScore W1845212532C71924100 @default.
- W1845212532 hasConceptScore W1845212532C84393581 @default.
- W1845212532 hasConceptScore W1845212532C86803240 @default.
- W1845212532 hasIssue "6" @default.
- W1845212532 hasLocation W18452125321 @default.
- W1845212532 hasLocation W18452125322 @default.
- W1845212532 hasLocation W18452125323 @default.
- W1845212532 hasLocation W18452125324 @default.
- W1845212532 hasOpenAccess W1845212532 @default.
- W1845212532 hasPrimaryLocation W18452125321 @default.
- W1845212532 hasRelatedWork W1977123261 @default.
- W1845212532 hasRelatedWork W2000944441 @default.
- W1845212532 hasRelatedWork W2044747643 @default.
- W1845212532 hasRelatedWork W2045449173 @default.
- W1845212532 hasRelatedWork W2047608102 @default.
- W1845212532 hasRelatedWork W2077998387 @default.
- W1845212532 hasRelatedWork W2088924510 @default.
- W1845212532 hasRelatedWork W2139586963 @default.
- W1845212532 hasRelatedWork W2418574610 @default.
- W1845212532 hasRelatedWork W4232349415 @default.
- W1845212532 hasVolume "45" @default.
- W1845212532 isParatext "false" @default.
- W1845212532 isRetracted "false" @default.
- W1845212532 magId "1845212532" @default.
- W1845212532 workType "article" @default.