Matches in SemOpenAlex for { <https://semopenalex.org/work/W1845408054> ?p ?o ?g. }
- W1845408054 endingPage "145" @default.
- W1845408054 startingPage "129" @default.
- W1845408054 abstract "Lipid rafts are micro-domains of ordered lipids (Lo phase) in biological membranes. The Lo phase of cellular membranes can be isolated from disordered lipids (Ld phase) after treatment with 1 % Triton X-100 at 4 °C in which the Lo phase forms the detergent-resistant membrane (DRM) fraction. The lipid composition of DRM derived from Madin-Darby canine kidney (MDCK) cells, McArdle cells and porcine sperm is compared with that of the whole cell. Remarkably, the unsaturation and chain length degree of aliphatic chains attached to phospholipids is virtually the same between DRM and whole cells. Cholesterol and sphingomyelin were enriched in DRMs but to a cell-specific molar ratio. Sulfatides (sphingolipids from MDCK cells) were enriched in the DRM while a seminolipid (an alkylacylglycerolipid from sperm) was depleted from the DRM. Treatment with <5 mM methyl-ß-cyclodextrin (MBCD) caused cholesterol removal from the DRM without affecting the composition and amount of the phospholipid while higher levels disrupted the DRM. The substantial amount of (poly)unsaturated phospholipids in DRMs as well as a low stoichiometric amount of cholesterol suggest that lipid rafts in biological membranes are more fluid and dynamic than previously anticipated. Using negative staining, ultrastructural features of DRM were monitored and in all three cell types the DRMs appeared as multi-lamellar vesicular structures with a similar morphology. The detergent resistance is a result of protein–cholesterol and sphingolipid interactions allowing a relatively passive attraction of phospholipids to maintain the Lo phase. For this special issue, the relevance of our findings is discussed in a sperm physiological context." @default.
- W1845408054 created "2016-06-24" @default.
- W1845408054 creator A5007392451 @default.
- W1845408054 creator A5040140423 @default.
- W1845408054 creator A5055147427 @default.
- W1845408054 creator A5077621578 @default.
- W1845408054 creator A5089445194 @default.
- W1845408054 date "2015-09-16" @default.
- W1845408054 modified "2023-09-25" @default.
- W1845408054 title "Ultrastructure and lipid composition of detergent-resistant membranes derived from mammalian sperm and two types of epithelial cells" @default.
- W1845408054 cites W1039301918 @default.
- W1845408054 cites W1533747850 @default.
- W1845408054 cites W1564861448 @default.
- W1845408054 cites W1583154040 @default.
- W1845408054 cites W1603398496 @default.
- W1845408054 cites W1603870224 @default.
- W1845408054 cites W1775749144 @default.
- W1845408054 cites W1896539835 @default.
- W1845408054 cites W1914926542 @default.
- W1845408054 cites W1964480443 @default.
- W1845408054 cites W1967816643 @default.
- W1845408054 cites W1972618613 @default.
- W1845408054 cites W1972706544 @default.
- W1845408054 cites W1975325447 @default.
- W1845408054 cites W1976608130 @default.
- W1845408054 cites W1977253956 @default.
- W1845408054 cites W1978281474 @default.
- W1845408054 cites W1980934355 @default.
- W1845408054 cites W1981914794 @default.
- W1845408054 cites W1982024352 @default.
- W1845408054 cites W1987032312 @default.
- W1845408054 cites W1988771171 @default.
- W1845408054 cites W1990394752 @default.
- W1845408054 cites W1991065572 @default.
- W1845408054 cites W1994363285 @default.
- W1845408054 cites W1994574697 @default.
- W1845408054 cites W1995622648 @default.
- W1845408054 cites W1998429632 @default.
- W1845408054 cites W2011529872 @default.
- W1845408054 cites W2013020652 @default.
- W1845408054 cites W2014742343 @default.
- W1845408054 cites W2016403157 @default.
- W1845408054 cites W2016689892 @default.
- W1845408054 cites W2018996953 @default.
- W1845408054 cites W2020523425 @default.
- W1845408054 cites W2021882008 @default.
- W1845408054 cites W2024134205 @default.
- W1845408054 cites W2025646685 @default.
- W1845408054 cites W2025894702 @default.
- W1845408054 cites W2027110836 @default.
- W1845408054 cites W2030317737 @default.
- W1845408054 cites W2032028794 @default.
- W1845408054 cites W2034122210 @default.
- W1845408054 cites W2035460630 @default.
- W1845408054 cites W2035707376 @default.
- W1845408054 cites W2036720638 @default.
- W1845408054 cites W2037735248 @default.
- W1845408054 cites W2038399376 @default.
- W1845408054 cites W2040124417 @default.
- W1845408054 cites W2041946821 @default.
- W1845408054 cites W2047815072 @default.
- W1845408054 cites W2052602536 @default.
- W1845408054 cites W2056112448 @default.
- W1845408054 cites W2057118881 @default.
- W1845408054 cites W2057558101 @default.
- W1845408054 cites W2060481671 @default.
- W1845408054 cites W2061333464 @default.
- W1845408054 cites W2065886456 @default.
- W1845408054 cites W2065984876 @default.
- W1845408054 cites W2069229446 @default.
- W1845408054 cites W2069466129 @default.
- W1845408054 cites W2070432888 @default.
- W1845408054 cites W2070468337 @default.
- W1845408054 cites W2075310427 @default.
- W1845408054 cites W2085239147 @default.
- W1845408054 cites W2087144997 @default.
- W1845408054 cites W2087378494 @default.
- W1845408054 cites W2089852404 @default.
- W1845408054 cites W2092111186 @default.
- W1845408054 cites W2092296126 @default.
- W1845408054 cites W2093402385 @default.
- W1845408054 cites W2095988962 @default.
- W1845408054 cites W2105505566 @default.
- W1845408054 cites W2106631104 @default.
- W1845408054 cites W2107411369 @default.
- W1845408054 cites W2108559196 @default.
- W1845408054 cites W2108797055 @default.
- W1845408054 cites W2117304280 @default.
- W1845408054 cites W2121936687 @default.
- W1845408054 cites W2124967367 @default.
- W1845408054 cites W2125041118 @default.
- W1845408054 cites W2127089274 @default.
- W1845408054 cites W2130575730 @default.
- W1845408054 cites W2133991651 @default.
- W1845408054 cites W2135371328 @default.
- W1845408054 cites W2136558879 @default.
- W1845408054 cites W2136795628 @default.
- W1845408054 cites W2137324750 @default.