Matches in SemOpenAlex for { <https://semopenalex.org/work/W18455514> ?p ?o ?g. }
- W18455514 endingPage "75" @default.
- W18455514 startingPage "63" @default.
- W18455514 abstract "In a previous report, the influence of the pH, the concentration, and the nature of the buffer on the retention and overloading behavior of propranolol (pKa = 9.45) was studied on Kromasil-C18 at 2.75 < pH < 6.75, using four buffers (phosphate, acetate, phthalate, and succinate), at three concentrations, 6, 20, and 60 mM. The results showed that the propranolol cation was eluted as an ion-pair with the buffer counter-anion. A similar study was carried out with Symmetry-C18 and Xterra-C18. Two additional buffers, formate and citrate, were also used. Propranolol elution band profiles were recorded for a small (less than 1 microg) and a large (375 microg) sample size. The results are similar to those obtained with Kromasil and confirm earlier conclusions. The buffer concentration, not its pH, controls the retention time of propranolol, in agreement with the chaotropic model. The retention factor depends also on the nature of the buffer, particularly on its valence, and on the hydrophobicity of the basic anion. With the monovalent anions HCOO- (pH 3.75), H2PO4- (pH 2.75), HOOC-Ph-COO- (pH 2.75), HOOC-CH2-CH2-COO- (pH 4.16), CH3COO- (pH 4.75) and HOOC-CHCOOH-COO- (pH 3.14), at moderate loadings, and for the two larger buffer concentrations, the band profiles are well accounted for by a simple bi-Langmuir isotherm model (no adsorbate-adsorbate interactions). By contrast, these profiles are accounted for by a bi-Moreau isotherm model (i.e., with significant adsorbate-adsorbate interactions) with the bivalent anions -OOC-Ph-COO- (pH 4.75), -OOC-CH2-CH2-COO- (pH 5.61), HPO4(2-) (pH 6.75), and HOOC-CHCOO(-)-COO- (pH 4.77) and with the trivalent anion -OOC-CHCOO(-)-COO- (pH 6.39). The best values of the isotherm parameters were determined using the inverse method. The saturation capacity and the equilibrium constant on the low-energy sites increase with increasing buffer concentration, a result consistent with the formation in the mobile phase of a hydrophobic complex between the propranolol cation and the buffer anion. With bivalent and trivalent anions, adsorbate-adsorbate interactions are strong on the low-energy sites but they remain negligible on the high-energy sites. The density of the high energy sites is lower and the equilibrium constant on the low-energy sites are both higher with the bivalent and the trivalent buffer anions than with the univalent buffer anions. These results are consistent with the formation of a 2:1 and a 3:1 propranolol-buffer complex with the bivalent and the trivalent anions, respectively." @default.
- W18455514 created "2016-06-24" @default.
- W18455514 creator A5038183935 @default.
- W18455514 creator A5089250649 @default.
- W18455514 date "2004-07-01" @default.
- W18455514 modified "2023-09-23" @default.
- W18455514 title "Effect of the pH, the concentration and the nature of the buffer on the adsorption mechanism of an ionic compound in reversed-phase liquid chromatography" @default.
- W18455514 cites W117429627 @default.
- W18455514 cites W1964801491 @default.
- W18455514 cites W1967096998 @default.
- W18455514 cites W1969272262 @default.
- W18455514 cites W1973750175 @default.
- W18455514 cites W1974475121 @default.
- W18455514 cites W1974758945 @default.
- W18455514 cites W1995433607 @default.
- W18455514 cites W1998554980 @default.
- W18455514 cites W1999701124 @default.
- W18455514 cites W1999922899 @default.
- W18455514 cites W2006713849 @default.
- W18455514 cites W2006905711 @default.
- W18455514 cites W2016277562 @default.
- W18455514 cites W2023261903 @default.
- W18455514 cites W2023653072 @default.
- W18455514 cites W2030446995 @default.
- W18455514 cites W2037321686 @default.
- W18455514 cites W2044359913 @default.
- W18455514 cites W2046150290 @default.
- W18455514 cites W2056739243 @default.
- W18455514 cites W2070373344 @default.
- W18455514 cites W2072231939 @default.
- W18455514 cites W2077152715 @default.
- W18455514 cites W2079602405 @default.
- W18455514 cites W2087027604 @default.
- W18455514 cites W2087710032 @default.
- W18455514 cites W2088890506 @default.
- W18455514 cites W2094405051 @default.
- W18455514 cites W2190070980 @default.
- W18455514 cites W2325784639 @default.
- W18455514 cites W4229854124 @default.
- W18455514 cites W4229989555 @default.
- W18455514 cites W4254806055 @default.
- W18455514 cites W4375230812 @default.
- W18455514 cites W46176729 @default.
- W18455514 doi "https://doi.org/10.1016/j.chroma.2004.05.004" @default.
- W18455514 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15281255" @default.
- W18455514 hasPublicationYear "2004" @default.
- W18455514 type Work @default.
- W18455514 sameAs 18455514 @default.
- W18455514 citedByCount "40" @default.
- W18455514 countsByYear W184555142012 @default.
- W18455514 countsByYear W184555142013 @default.
- W18455514 countsByYear W184555142014 @default.
- W18455514 countsByYear W184555142015 @default.
- W18455514 countsByYear W184555142016 @default.
- W18455514 countsByYear W184555142018 @default.
- W18455514 countsByYear W184555142019 @default.
- W18455514 countsByYear W184555142022 @default.
- W18455514 crossrefType "journal-article" @default.
- W18455514 hasAuthorship W18455514A5038183935 @default.
- W18455514 hasAuthorship W18455514A5089250649 @default.
- W18455514 hasConcept C113196181 @default.
- W18455514 hasConcept C147789679 @default.
- W18455514 hasConcept C150394285 @default.
- W18455514 hasConcept C161790260 @default.
- W18455514 hasConcept C17525397 @default.
- W18455514 hasConcept C178790620 @default.
- W18455514 hasConcept C179104552 @default.
- W18455514 hasConcept C184235292 @default.
- W18455514 hasConcept C184651966 @default.
- W18455514 hasConcept C185592680 @default.
- W18455514 hasConcept C187428577 @default.
- W18455514 hasConcept C2778117296 @default.
- W18455514 hasConcept C2779933727 @default.
- W18455514 hasConcept C2780178953 @default.
- W18455514 hasConcept C43617362 @default.
- W18455514 hasConcept C55493867 @default.
- W18455514 hasConcept C68801617 @default.
- W18455514 hasConcept C73826308 @default.
- W18455514 hasConceptScore W18455514C113196181 @default.
- W18455514 hasConceptScore W18455514C147789679 @default.
- W18455514 hasConceptScore W18455514C150394285 @default.
- W18455514 hasConceptScore W18455514C161790260 @default.
- W18455514 hasConceptScore W18455514C17525397 @default.
- W18455514 hasConceptScore W18455514C178790620 @default.
- W18455514 hasConceptScore W18455514C179104552 @default.
- W18455514 hasConceptScore W18455514C184235292 @default.
- W18455514 hasConceptScore W18455514C184651966 @default.
- W18455514 hasConceptScore W18455514C185592680 @default.
- W18455514 hasConceptScore W18455514C187428577 @default.
- W18455514 hasConceptScore W18455514C2778117296 @default.
- W18455514 hasConceptScore W18455514C2779933727 @default.
- W18455514 hasConceptScore W18455514C2780178953 @default.
- W18455514 hasConceptScore W18455514C43617362 @default.
- W18455514 hasConceptScore W18455514C55493867 @default.
- W18455514 hasConceptScore W18455514C68801617 @default.
- W18455514 hasConceptScore W18455514C73826308 @default.
- W18455514 hasIssue "1-2" @default.
- W18455514 hasLocation W184555141 @default.