FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1848203461> ?p ?o ?g. }

• W1848203461 endingPage "413" @default.
• W1848203461 startingPage "403" @default.
• W1848203461 abstract "ABSTRACT The luminal and basal epithelial cells in the human mammary gland can be distinguished in tissue sections on the basis of the pattern of keratins they express. Moreover, the invasive cells in primary carcinomas show a keratin profile that corresponds to that of the dominant luminal cell (7, 8, 18, 19). When homogeneous populations of luminal epithelial cells from milk or from breast cancer metastases are cultured the profile of keratin expression seen in vivo is maintained. We have therefore used monospecific antibodies reactive with individual keratins to examine the phenotype of cells cultured in three different media from reduction mammoplasty tissue that contains both luminal and basal cells. The phenotype of cells cultured from primary breast cancers in one of these media (MCDB 170) has also been examined. In characterizing cell phenotypes, antibodies to a polymorphic epithelial mucin (PEM) expressed in vivo by luminal cells, and to smooth muscle (a) actin, expressed in vivo by basal cells, have also been used. Our results show that proliferation of different cell phenotypes is selected for in different media. In milk mix (MX) developed for growth of luminal cells from milk, only the luminal cell phenotype proliferates (for only 1 or 2 passages). In medium MCDB 170, which was developed for long-term growth of human mammary epithelial cells from reduction mammoplasty organoids, cells from the basal layer proliferate, while in MM medium the basal phenotype dominates, but a few cells with the luminal phenotype are found. Around passage 3, in medium MCDB 170, most cells senesce and a subpopulation of cells proliferates on further passage. These cells retain expression of the basal epithelial keratins but also express some features characteristic of luminal epithelial cells, suggesting that the basal layer may contain a stem cell that can develop along the luminal lineage. In culture, however, they do not express keratin 19, which in vivo is a feature of the fully differentiated luminal cell. The cells cultured from primary breast cancer in medium MCDB 170 have a similar keratin profile to that of the normal cells cultured in this medium. They do not express keratin 19, even though the invasive cells in primary cancers homogeneously express this keratin in vivo. The invasive phenotype, which in its keratin profile corresponds to the differentiated luminal cell and that of the metastatic cancer lines, cannot be cultured from primary breast cancers using MX, which supports proliferation of the corresponding normal cell." @default.
• W1848203461 created "2016-06-24" @default.
• W1848203461 creator A5008688636 @default.
• W1848203461 creator A5014197662 @default.
• W1848203461 creator A5015713295 @default.
• W1848203461 creator A5019285825 @default.
• W1848203461 creator A5038528339 @default.
• W1848203461 creator A5044235089 @default.
• W1848203461 creator A5090729430 @default.
• W1848203461 date "1989-11-01" @default.
• W1848203461 modified "2023-10-16" @default.
• W1848203461 title "Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to <i>in vivo</i> phenotypes and influence of medium" @default.
• W1848203461 cites W1541668845 @default.
• W1848203461 cites W1953511300 @default.
• W1848203461 cites W1963735070 @default.
• W1848203461 cites W1968467528 @default.
• W1848203461 cites W1980429073 @default.
• W1848203461 cites W1980645442 @default.
• W1848203461 cites W1984502321 @default.
• W1848203461 cites W1989057019 @default.
• W1848203461 cites W1989070853 @default.
• W1848203461 cites W1989995458 @default.
• W1848203461 cites W2002263141 @default.
• W1848203461 cites W2003113478 @default.
• W1848203461 cites W2004277017 @default.
• W1848203461 cites W2007461809 @default.
• W1848203461 cites W2013681211 @default.
• W1848203461 cites W2016188858 @default.
• W1848203461 cites W2016693266 @default.
• W1848203461 cites W2019994666 @default.
• W1848203461 cites W2020333197 @default.
• W1848203461 cites W2028211962 @default.
• W1848203461 cites W2029453803 @default.
• W1848203461 cites W2047719732 @default.
• W1848203461 cites W2059406629 @default.
• W1848203461 cites W2060916135 @default.
• W1848203461 cites W2061325267 @default.
• W1848203461 cites W2070624699 @default.
• W1848203461 cites W2076124713 @default.
• W1848203461 cites W2080532933 @default.
• W1848203461 cites W2088143614 @default.
• W1848203461 cites W2100905639 @default.
• W1848203461 cites W2108283430 @default.
• W1848203461 cites W2122153903 @default.
• W1848203461 cites W2125880960 @default.
• W1848203461 cites W2140905177 @default.
• W1848203461 cites W2162422287 @default.
• W1848203461 cites W2165751673 @default.
• W1848203461 cites W2170202796 @default.
• W1848203461 cites W2294334808 @default.
• W1848203461 cites W2316200332 @default.
• W1848203461 cites W2441746706 @default.
• W1848203461 cites W2475402469 @default.
• W1848203461 cites W4322701374 @default.
• W1848203461 doi "https://doi.org/10.1242/jcs.94.3.403" @default.
• W1848203461 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2483723" @default.
• W1848203461 hasPublicationYear "1989" @default.
• W1848203461 type Work @default.
• W1848203461 sameAs 1848203461 @default.
• W1848203461 citedByCount "232" @default.
• W1848203461 countsByYear W18482034612012 @default.
• W1848203461 countsByYear W18482034612013 @default.
• W1848203461 countsByYear W18482034612014 @default.
• W1848203461 countsByYear W18482034612015 @default.
• W1848203461 countsByYear W18482034612016 @default.
• W1848203461 countsByYear W18482034612017 @default.
• W1848203461 countsByYear W18482034612018 @default.
• W1848203461 countsByYear W18482034612019 @default.
• W1848203461 countsByYear W18482034612020 @default.
• W1848203461 countsByYear W18482034612021 @default.
• W1848203461 countsByYear W18482034612022 @default.
• W1848203461 countsByYear W18482034612023 @default.
• W1848203461 crossrefType "journal-article" @default.
• W1848203461 hasAuthorship W1848203461A5008688636 @default.
• W1848203461 hasAuthorship W1848203461A5014197662 @default.
• W1848203461 hasAuthorship W1848203461A5015713295 @default.
• W1848203461 hasAuthorship W1848203461A5019285825 @default.
• W1848203461 hasAuthorship W1848203461A5038528339 @default.
• W1848203461 hasAuthorship W1848203461A5044235089 @default.
• W1848203461 hasAuthorship W1848203461A5090729430 @default.
• W1848203461 hasConcept C104317684 @default.
• W1848203461 hasConcept C121608353 @default.
• W1848203461 hasConcept C127716648 @default.
• W1848203461 hasConcept C142724271 @default.
• W1848203461 hasConcept C1491633281 @default.
• W1848203461 hasConcept C150903083 @default.
• W1848203461 hasConcept C151730666 @default.
• W1848203461 hasConcept C179264091 @default.
• W1848203461 hasConcept C189014844 @default.
• W1848203461 hasConcept C207001950 @default.
• W1848203461 hasConcept C2778001805 @default.
• W1848203461 hasConcept C529295009 @default.
• W1848203461 hasConcept C530470458 @default.
• W1848203461 hasConcept C54355233 @default.
• W1848203461 hasConcept C55493867 @default.
• W1848203461 hasConcept C71924100 @default.
• W1848203461 hasConcept C81241287 @default.
• W1848203461 hasConcept C81885089 @default.

• next