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• W1849146844 endingPage "1700" @default.
• W1849146844 startingPage "1689" @default.
• W1849146844 abstract "Abstract We report the isolation of phoB and phoU mutants of the bacterium Rhizobium (Sinorhizobium) meliloti. These mutants form N2-fixing nodules on the roots of alfalfa plants. R. meliloti mutants defective in the phoCDET (ndvF) encoded phosphate transport system grow slowly in media containing 2 mm Pi, and form nodules which fail to fix nitrogen (Fix−). We show that the transfer of phoB or phoU insertion mutations into phoC mutant strains restores the ability of these mutants to: (i) form normal N2-fixing root-nodules, and (ii) grow like the wild type in media containing 2 mm Pi. We also show that expression of the alternate orfA pit encoded Pi transport system is negatively regulated by the phoB gene product, whereas phoB is required for phoCDET expression. We suggest that in R. meliloti cells growing under Pi limiting conditions, PhoB protein activates phoCDET transcription and represses orfA pit transcription. Our results suggest that there are major differences between the Escherichia coli and R. meliloti phosphate regulatory systems." @default.
• W1849146844 created "2016-06-24" @default.
• W1849146844 creator A5000015314 @default.
• W1849146844 creator A5039259726 @default.
• W1849146844 date "1998-04-01" @default.
• W1849146844 modified "2023-09-26" @default.
• W1849146844 title "Regulation of Phosphate Assimilation in Rhizobium (Sinorhizobium) meliloti" @default.
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• W1849146844 doi "https://doi.org/10.1093/genetics/148.4.1689" @default.
• W1849146844 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1460103" @default.
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• W1849146844 hasPublicationYear "1998" @default.
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