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- W1850192067 abstract "ABSTRACT To facilitate a quantitative morphological analysis of early mouse development under controlled conditions, a perfusion culture system capable of supporting embryogenesis to blastocyst stage has been developed. The use of a mesh system allows identification of individual embryos by position, and control of their orientation during culture and preparation for light and electron microscopy. Quantitative evaluation of tissue-processing procedures has permitted selection of conditions which reduce changes in linear dimensions to –1·6 ± 1·8% in two-cell embryos. Through the definition of a coordinate system based on mesh structure and the development of a special sectioning procedure, sections can be localized within the intact embryo and three-dimensional coordinates given to any element of embryo volume." @default.
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- W1850192067 date "1977-08-01" @default.
- W1850192067 modified "2023-09-23" @default.
- W1850192067 title "Quantitative morphological analysis of early mouseembryogenesis <i>in vitro</i>:I. Perfusion culture system, tissue preparation, sampling" @default.
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- W1850192067 doi "https://doi.org/10.1242/dev.40.1.91" @default.
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