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• W1850964633 abstract "Eukaryotic protein kinases phosphorylate substrates at serine, threonine, and tyrosine residues that fall within the context of short sequence motifs. Knowing the phosphorylation site motif for a protein kinase facilitates designing substrates for kinase assays and mapping phosphorylation sites in protein substrates. Here, we describe an arrayed peptide library protocol for rapidly determining kinase phosphorylation consensus sequences. This method uses a set of peptide mixtures in which each of the 20 amino acid residues is systematically substituted at nine positions surrounding a central site of phosphorylation. Peptide mixtures are arrayed in multiwell plates and analyzed by radiolabel assay with the kinase of interest. The preferred sequence is determined from the relative rate of phosphorylation of each peptide in the array. Consensus peptides based on these sequences typically serve as efficient and specific kinase substrates for high-throughput screening or incorporation into biosensors." @default.
• W1850964633 created "2016-06-24" @default.
• W1850964633 creator A5041859681 @default.
• W1850964633 creator A5045477429 @default.
• W1850964633 date "2016-01-01" @default.
• W1850964633 modified "2023-10-18" @default.
• W1850964633 title "Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide Libraries" @default.
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• W1850964633 doi "https://doi.org/10.1007/978-1-4939-3073-9_15" @default.
• W1850964633 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4784478" @default.
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