FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1851831207> ?p ?o ?g. }

• W1851831207 abstract "WNT5A is an extracellular glycoprotein that activates several Wnt signaling pathways important in cancer. Significantly, Wnt5a expression is altered in numerous cancers. Little is known about Wnt5a gene regulation but current data indicate that misregulation of Wnt5a expression involves non genetic changes. The goal of this study was to characterize transcriptional regulation from two alternate Wnt5a promoters, A and B. To analyze the relative level of expression from the human Wnt5a alternative promoters, promoter-luciferase reporter constructs containing different amounts of upstream sequence from promoters A and B were transiently transfected into NIH3T3 and Caco-2 cell lines. The relative level of promoter activity was compared by measuring luciferase activity in the transfected cells. Both Wnt5a promoters A and B were functional but found to have differential expression patterns in NIH3T3 and Caco-2 cells, indicating both positive and negative regulatory sequences. The transcription factor NFkappaB and the MAPK signaling pathway were studied to determine if they influence the transcriptional activity of Wnt5a promoters A and B. Stable lines of NIH3T3 cells with Wnt5a promoters A and B were treated for 6 and 24 hours with TNFalpha, a known inducer of NFkappaB activity, and inhibitors of MAPK pathway kinases (MEK1/2 and ERK). The cells were collected and assayed for firefly luciferase activity (relative light units) and standardized to DNA content. TNFalpha slightly increased promoters A and B activity at 6 hrs. TNFalpha had little or no effect on promoter A at 24 hours, whereas promoter B activity increased between 1.28 and 2.84 fold. The NFkappaB inhibitor, JSH-23, confirmed that NFkappaB is involved in the response of promoter B but not promoter A to TNFalpha. MEK1/2 inhibitor had inconsistent effects on promoter A, whereas promoter B activity decreases at both time points. ERK inhibitor increased promoter A activity for both 6 and 24 hours, whereas activity only increased for promoter B at 24 hours. These findings were further examined by measuring the relative contribution of Wnt5a alternative promoter A and B endogenous transcripts in both NIH3T3 and GM03349. Custom primers that amplify and detect human and mouse Wnt5a promoters A and B specific transcripts were created to analyze the relative amount of each transcript by qRT-PCR. Results show that promoter A generates more transcripts than promoter B and that TNFalpha increases the activity of both promoters in mouse and human fibroblasts. Overall, these data indicate that Wnt5a promoters A and B are differentially regulated and that NFkappaB influences transcriptional activity of Wnt5a promoter B rather than promoter A." @default.
• W1851831207 created "2016-06-24" @default.
• W1851831207 creator A5036160916 @default.
• W1851831207 date "2011-01-01" @default.
• W1851831207 modified "2023-09-27" @default.
• W1851831207 title "Characterization and regulation of Wnt5a alternate promoters A and B" @default.
• W1851831207 cites W127737003 @default.
• W1851831207 cites W1775301645 @default.
• W1851831207 cites W1971242931 @default.
• W1851831207 cites W1982935330 @default.
• W1851831207 cites W2004170013 @default.
• W1851831207 cites W2006037174 @default.
• W1851831207 cites W2006418914 @default.
• W1851831207 cites W2017270956 @default.
• W1851831207 cites W2023186669 @default.
• W1851831207 cites W2040467756 @default.
• W1851831207 cites W2041463373 @default.
• W1851831207 cites W2041850471 @default.
• W1851831207 cites W2062730435 @default.
• W1851831207 cites W2063501462 @default.
• W1851831207 cites W2064988924 @default.
• W1851831207 cites W2071642351 @default.
• W1851831207 cites W2073797959 @default.
• W1851831207 cites W2088452641 @default.
• W1851831207 cites W2098067206 @default.
• W1851831207 cites W2099011309 @default.
• W1851831207 cites W2104537787 @default.
• W1851831207 cites W2132884161 @default.
• W1851831207 cites W2146455000 @default.
• W1851831207 cites W2155002380 @default.
• W1851831207 cites W2168593012 @default.
• W1851831207 cites W2168951140 @default.
• W1851831207 cites W2409599271 @default.
• W1851831207 hasPublicationYear "2011" @default.
• W1851831207 type Work @default.
• W1851831207 sameAs 1851831207 @default.
• W1851831207 citedByCount "0" @default.
• W1851831207 crossrefType "journal-article" @default.
• W1851831207 hasAuthorship W1851831207A5036160916 @default.
• W1851831207 hasConcept C101762097 @default.
• W1851831207 hasConcept C104317684 @default.
• W1851831207 hasConcept C111335760 @default.
• W1851831207 hasConcept C137620995 @default.
• W1851831207 hasConcept C150194340 @default.
• W1851831207 hasConcept C153911025 @default.
• W1851831207 hasConcept C165864922 @default.
• W1851831207 hasConcept C54009773 @default.
• W1851831207 hasConcept C54355233 @default.
• W1851831207 hasConcept C57074206 @default.
• W1851831207 hasConcept C62478195 @default.
• W1851831207 hasConcept C86339819 @default.
• W1851831207 hasConcept C86803240 @default.
• W1851831207 hasConcept C95444343 @default.
• W1851831207 hasConceptScore W1851831207C101762097 @default.
• W1851831207 hasConceptScore W1851831207C104317684 @default.
• W1851831207 hasConceptScore W1851831207C111335760 @default.
• W1851831207 hasConceptScore W1851831207C137620995 @default.
• W1851831207 hasConceptScore W1851831207C150194340 @default.
• W1851831207 hasConceptScore W1851831207C153911025 @default.
• W1851831207 hasConceptScore W1851831207C165864922 @default.
• W1851831207 hasConceptScore W1851831207C54009773 @default.
• W1851831207 hasConceptScore W1851831207C54355233 @default.
• W1851831207 hasConceptScore W1851831207C57074206 @default.
• W1851831207 hasConceptScore W1851831207C62478195 @default.
• W1851831207 hasConceptScore W1851831207C86339819 @default.
• W1851831207 hasConceptScore W1851831207C86803240 @default.
• W1851831207 hasConceptScore W1851831207C95444343 @default.
• W1851831207 hasLocation W18518312071 @default.
• W1851831207 hasOpenAccess W1851831207 @default.
• W1851831207 hasPrimaryLocation W18518312071 @default.
• W1851831207 hasRelatedWork W1499666737 @default.
• W1851831207 hasRelatedWork W158273144 @default.
• W1851831207 hasRelatedWork W1597819373 @default.
• W1851831207 hasRelatedWork W1976720178 @default.
• W1851831207 hasRelatedWork W1981832308 @default.
• W1851831207 hasRelatedWork W1987166459 @default.
• W1851831207 hasRelatedWork W1999362894 @default.
• W1851831207 hasRelatedWork W2041011733 @default.
• W1851831207 hasRelatedWork W2048253789 @default.
• W1851831207 hasRelatedWork W2075939587 @default.
• W1851831207 hasRelatedWork W2083609595 @default.
• W1851831207 hasRelatedWork W2086179020 @default.
• W1851831207 hasRelatedWork W2091053063 @default.
• W1851831207 hasRelatedWork W2145732753 @default.
• W1851831207 hasRelatedWork W2163751507 @default.
• W1851831207 hasRelatedWork W2283079036 @default.
• W1851831207 hasRelatedWork W2403485614 @default.
• W1851831207 hasRelatedWork W2891953191 @default.
• W1851831207 hasRelatedWork W3038825876 @default.
• W1851831207 hasRelatedWork W801142006 @default.
• W1851831207 isParatext "false" @default.
• W1851831207 isRetracted "false" @default.
• W1851831207 magId "1851831207" @default.
• W1851831207 workType "article" @default.