Matches in SemOpenAlex for { <https://semopenalex.org/work/W1851888085> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W1851888085 endingPage "13" @default.
- W1851888085 startingPage "8" @default.
- W1851888085 abstract "Gentamicin (Gent) is an aminoglycoside antibiotic being used in livestock sector. Gent residues could cause some genetic disorders by nonsense mutations. This study aimed to develop IgY-based ELISA for the detection of Gent in animal products. Gent was conjugated with Bovine serum albumin (BSA) by carbodiimide method for further immunization in the laying chickens. PEG-6000 extraction method was employed to extract IgY from the egg yolk. The titer of anti-Gent-IgY attained the peak of 1:256,000 after the 5(th) booster immunization. Checkerboard titration confirmed that, anti-Gent IgY in 1:2,000 dilution could give an Optical Density (OD) 1.0 at 2 µg mL(-1) of Gent-OVA coating concentration. IgY-based indirect competitive ELISA (Ic-ELISA) showed that, the IC50 value of anti-Gent IgY was 2.69 ng mL(-1) and regression curve equation was y = -16.27x + 56.97 (R(2) = 0.95, n = 3), confirming that, the detection limit (LOD, IC10 value) was 0.01 ng mL(-1). Recoveries from fresh milk, pork and chicken samples were ranged from 69.82% to 94.32%, with relative standard deviation lower than 10.88%. Our results suggested that generated anti-Gent IgY antibodies can be used in routine screening analysis of Gent residues in food samples." @default.
- W1851888085 created "2016-06-24" @default.
- W1851888085 creator A5000636451 @default.
- W1851888085 creator A5015416603 @default.
- W1851888085 creator A5049088969 @default.
- W1851888085 creator A5072074829 @default.
- W1851888085 creator A5082213642 @default.
- W1851888085 creator A5090522057 @default.
- W1851888085 date "2015-10-29" @default.
- W1851888085 modified "2023-09-27" @default.
- W1851888085 title "Development of indirect competitive ELISA using egg yolk-derived immunoglobulin (IgY) for the detection of Gentamicin residues" @default.
- W1851888085 cites W113781936 @default.
- W1851888085 cites W125820677 @default.
- W1851888085 cites W1518439833 @default.
- W1851888085 cites W1972070745 @default.
- W1851888085 cites W1977078199 @default.
- W1851888085 cites W1984443949 @default.
- W1851888085 cites W1997429802 @default.
- W1851888085 cites W2014508375 @default.
- W1851888085 cites W2031599199 @default.
- W1851888085 cites W2048477555 @default.
- W1851888085 cites W2060954344 @default.
- W1851888085 cites W2065964744 @default.
- W1851888085 cites W2066008460 @default.
- W1851888085 cites W2088378849 @default.
- W1851888085 cites W2088761725 @default.
- W1851888085 cites W2153790548 @default.
- W1851888085 cites W2313242299 @default.
- W1851888085 cites W4236997449 @default.
- W1851888085 cites W73898233 @default.
- W1851888085 doi "https://doi.org/10.1080/03601234.2015.1080479" @default.
- W1851888085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26513166" @default.
- W1851888085 hasPublicationYear "2015" @default.
- W1851888085 type Work @default.
- W1851888085 sameAs 1851888085 @default.
- W1851888085 citedByCount "20" @default.
- W1851888085 countsByYear W18518880852016 @default.
- W1851888085 countsByYear W18518880852017 @default.
- W1851888085 countsByYear W18518880852018 @default.
- W1851888085 countsByYear W18518880852019 @default.
- W1851888085 countsByYear W18518880852020 @default.
- W1851888085 countsByYear W18518880852021 @default.
- W1851888085 countsByYear W18518880852022 @default.
- W1851888085 countsByYear W18518880852023 @default.
- W1851888085 crossrefType "journal-article" @default.
- W1851888085 hasAuthorship W1851888085A5000636451 @default.
- W1851888085 hasAuthorship W1851888085A5015416603 @default.
- W1851888085 hasAuthorship W1851888085A5049088969 @default.
- W1851888085 hasAuthorship W1851888085A5072074829 @default.
- W1851888085 hasAuthorship W1851888085A5082213642 @default.
- W1851888085 hasAuthorship W1851888085A5090522057 @default.
- W1851888085 hasConcept C119128265 @default.
- W1851888085 hasConcept C159654299 @default.
- W1851888085 hasConcept C185592680 @default.
- W1851888085 hasConcept C203014093 @default.
- W1851888085 hasConcept C2775832221 @default.
- W1851888085 hasConcept C2778951572 @default.
- W1851888085 hasConcept C2781321622 @default.
- W1851888085 hasConcept C31903555 @default.
- W1851888085 hasConcept C32611913 @default.
- W1851888085 hasConcept C43617362 @default.
- W1851888085 hasConcept C501593827 @default.
- W1851888085 hasConcept C55493867 @default.
- W1851888085 hasConcept C86803240 @default.
- W1851888085 hasConceptScore W1851888085C119128265 @default.
- W1851888085 hasConceptScore W1851888085C159654299 @default.
- W1851888085 hasConceptScore W1851888085C185592680 @default.
- W1851888085 hasConceptScore W1851888085C203014093 @default.
- W1851888085 hasConceptScore W1851888085C2775832221 @default.
- W1851888085 hasConceptScore W1851888085C2778951572 @default.
- W1851888085 hasConceptScore W1851888085C2781321622 @default.
- W1851888085 hasConceptScore W1851888085C31903555 @default.
- W1851888085 hasConceptScore W1851888085C32611913 @default.
- W1851888085 hasConceptScore W1851888085C43617362 @default.
- W1851888085 hasConceptScore W1851888085C501593827 @default.
- W1851888085 hasConceptScore W1851888085C55493867 @default.
- W1851888085 hasConceptScore W1851888085C86803240 @default.
- W1851888085 hasIssue "1" @default.
- W1851888085 hasLocation W18518880851 @default.
- W1851888085 hasLocation W18518880852 @default.
- W1851888085 hasOpenAccess W1851888085 @default.
- W1851888085 hasPrimaryLocation W18518880851 @default.
- W1851888085 hasRelatedWork W1599770027 @default.
- W1851888085 hasRelatedWork W2003691832 @default.
- W1851888085 hasRelatedWork W2050075993 @default.
- W1851888085 hasRelatedWork W2330330487 @default.
- W1851888085 hasRelatedWork W2357994263 @default.
- W1851888085 hasRelatedWork W2371433017 @default.
- W1851888085 hasRelatedWork W2381991529 @default.
- W1851888085 hasRelatedWork W2382070577 @default.
- W1851888085 hasRelatedWork W2387377172 @default.
- W1851888085 hasRelatedWork W2940950100 @default.
- W1851888085 hasVolume "51" @default.
- W1851888085 isParatext "false" @default.
- W1851888085 isRetracted "false" @default.
- W1851888085 magId "1851888085" @default.
- W1851888085 workType "article" @default.