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- W185263678 abstract "Dear Editors, The urease ELISA assay is based on a pH change which occurs when urease-conjugated anti- body reacts with a urea substrate-releasing am- monia (Chandler et al., 1982). The distinctive yel- low to purple colour change in the bromocresol purple indicator means that the assay can be read either by eye or by means of an ELISA plate reader. Recently Lo et al. (1988) demonstrated that the urease ELISA was prone to false positive reactions which occurred when ammonia pro- duced in one well was transferred in aerosols to adjacent wells during the incubation time required for the reaction to develop. This finding, together with some inconsistent results with putative H-Y antisera in our laboratory, prompted us to carry out a detailed investigation of the assay system as it pertained to H-Y serology. The H-Y antigen is a widely conserved minor histocompatibility antigen that characterises the heterogametic sex, i.e., males in mammals, and females in birds and fishes (Nakamura et al., 1987). T cell immune respon- siveness to the H-Y antigen has been well docu- mented (Simpson, 1982), but since the first report of a polyclonal antibody to this antigen (Goldberg" @default.
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- W185263678 date "1990-04-01" @default.
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- W185263678 title "The urease ELISA for H-Y antibody" @default.
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- W185263678 doi "https://doi.org/10.1016/0022-1759(90)90223-i" @default.
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