FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1852802315> ?p ?o ?g. }

• W1852802315 abstract "Jembrana disease virus (JDV) is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. An easy and rapid diagnostic method is essential for further control this disease. We used a reverse transcription loop-mediated isothermal amplification (RT-LAMP) combined with lateral flow dipstick (LFD), based on conserved tm subunit of Jembrana disease virus env gene. The RT-LAMP conditions were optimized by varying the concentration of MgSO4, betaine, dNTP, and temperature as well as the time and duration of reaction. The primers sensitivity for JDV was confirmed. The method was able to detect env-tm gene dilution which contained 2 × 10(-15) g of template. Comparatively, the sensitivity of RT-LAMP/LFD was 100-fold more sensitive than reverse transcription-polymerase chain reaction. The primers specificity for JDV was also confirmed using positive and negative controls. This work also showed that virus detection could be done not only on total RNA extracted from blood but various organs could also be analyzed for the presence of JDV using RT-LAMP/LFD method. The whole process, including the LAMP reaction and the LFD hybridization step only lasts approximately 75 min. Results of analysis can be easily observed with naked eyes without addition of any chemical or further analysis. The combination of RT-LAMP with LFD makes the method a more suitable diagnostic tool in conditions where sophisticated and expensive equipments are not available for field investigations on Jembrana disease in Bali cattle." @default.
• W1852802315 created "2016-06-24" @default.
• W1852802315 creator A5011477501 @default.
• W1852802315 creator A5047358782 @default.
• W1852802315 creator A5061710342 @default.
• W1852802315 creator A5068789143 @default.
• W1852802315 creator A5078809273 @default.
• W1852802315 creator A5080633606 @default.
• W1852802315 creator A5082227440 @default.
• W1852802315 date "2015-08-26" @default.
• W1852802315 modified "2023-10-01" @default.
• W1852802315 title "Use of reverse transcription loop-mediated isothermal amplification combined with lateral flow dipstick for an easy and rapid detection of Jembrana disease virus" @default.
• W1852802315 cites W1975801865 @default.
• W1852802315 cites W1979690819 @default.
• W1852802315 cites W1981561233 @default.
• W1852802315 cites W1986257828 @default.
• W1852802315 cites W1989331572 @default.
• W1852802315 cites W1997153901 @default.
• W1852802315 cites W2036415277 @default.
• W1852802315 cites W2037557838 @default.
• W1852802315 cites W2039992260 @default.
• W1852802315 cites W2055212432 @default.
• W1852802315 cites W2069957647 @default.
• W1852802315 cites W2097174592 @default.
• W1852802315 cites W2100718925 @default.
• W1852802315 cites W2105275554 @default.
• W1852802315 cites W2106239188 @default.
• W1852802315 cites W2141279039 @default.
• W1852802315 cites W2142135874 @default.
• W1852802315 cites W2149136689 @default.
• W1852802315 cites W2159130128 @default.
• W1852802315 doi "https://doi.org/10.1007/s13337-015-0277-5" @default.
• W1852802315 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4571594" @default.
• W1852802315 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26396986" @default.
• W1852802315 hasPublicationYear "2015" @default.
• W1852802315 type Work @default.
• W1852802315 sameAs 1852802315 @default.
• W1852802315 citedByCount "3" @default.
• W1852802315 countsByYear W18528023152015 @default.
• W1852802315 countsByYear W18528023152018 @default.
• W1852802315 countsByYear W18528023152019 @default.
• W1852802315 crossrefType "journal-article" @default.
• W1852802315 hasAuthorship W1852802315A5011477501 @default.
• W1852802315 hasAuthorship W1852802315A5047358782 @default.
• W1852802315 hasAuthorship W1852802315A5061710342 @default.
• W1852802315 hasAuthorship W1852802315A5068789143 @default.
• W1852802315 hasAuthorship W1852802315A5078809273 @default.
• W1852802315 hasAuthorship W1852802315A5080633606 @default.
• W1852802315 hasAuthorship W1852802315A5082227440 @default.
• W1852802315 hasBestOaLocation W18528023152 @default.
• W1852802315 hasConcept C104317684 @default.
• W1852802315 hasConcept C105580179 @default.
• W1852802315 hasConcept C138885662 @default.
• W1852802315 hasConcept C153911025 @default.
• W1852802315 hasConcept C156719811 @default.
• W1852802315 hasConcept C159047783 @default.
• W1852802315 hasConcept C179926584 @default.
• W1852802315 hasConcept C2522874641 @default.
• W1852802315 hasConcept C2780026642 @default.
• W1852802315 hasConcept C2780327821 @default.
• W1852802315 hasConcept C2780608908 @default.
• W1852802315 hasConcept C41895202 @default.
• W1852802315 hasConcept C4692481 @default.
• W1852802315 hasConcept C49105822 @default.
• W1852802315 hasConcept C54355233 @default.
• W1852802315 hasConcept C552990157 @default.
• W1852802315 hasConcept C55493867 @default.
• W1852802315 hasConcept C60635243 @default.
• W1852802315 hasConcept C86803240 @default.
• W1852802315 hasConceptScore W1852802315C104317684 @default.
• W1852802315 hasConceptScore W1852802315C105580179 @default.
• W1852802315 hasConceptScore W1852802315C138885662 @default.
• W1852802315 hasConceptScore W1852802315C153911025 @default.
• W1852802315 hasConceptScore W1852802315C156719811 @default.
• W1852802315 hasConceptScore W1852802315C159047783 @default.
• W1852802315 hasConceptScore W1852802315C179926584 @default.
• W1852802315 hasConceptScore W1852802315C2522874641 @default.
• W1852802315 hasConceptScore W1852802315C2780026642 @default.
• W1852802315 hasConceptScore W1852802315C2780327821 @default.
• W1852802315 hasConceptScore W1852802315C2780608908 @default.
• W1852802315 hasConceptScore W1852802315C41895202 @default.
• W1852802315 hasConceptScore W1852802315C4692481 @default.
• W1852802315 hasConceptScore W1852802315C49105822 @default.
• W1852802315 hasConceptScore W1852802315C54355233 @default.
• W1852802315 hasConceptScore W1852802315C552990157 @default.
• W1852802315 hasConceptScore W1852802315C55493867 @default.
• W1852802315 hasConceptScore W1852802315C60635243 @default.
• W1852802315 hasConceptScore W1852802315C86803240 @default.
• W1852802315 hasLocation W18528023151 @default.
• W1852802315 hasLocation W18528023152 @default.
• W1852802315 hasLocation W18528023153 @default.
• W1852802315 hasLocation W18528023154 @default.
• W1852802315 hasOpenAccess W1852802315 @default.
• W1852802315 hasPrimaryLocation W18528023151 @default.
• W1852802315 hasRelatedWork W1564818937 @default.
• W1852802315 hasRelatedWork W1968170805 @default.
• W1852802315 hasRelatedWork W1971038971 @default.
• W1852802315 hasRelatedWork W1995664273 @default.
• W1852802315 hasRelatedWork W2044912982 @default.
• W1852802315 hasRelatedWork W2045983583 @default.

• next