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• W1853643611 abstract "Research Article1 February 1990free access Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysis. A.L. Roldan A.L. Roldan University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author M.V. Cubellis M.V. Cubellis University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author M.T. Masucci M.T. Masucci University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author N. Behrendt N. Behrendt University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author L.R. Lund L.R. Lund University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author K. Danø K. Danø University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author E. Appella E. Appella University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author F. Blasi F. Blasi University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author A.L. Roldan A.L. Roldan University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author M.V. Cubellis M.V. Cubellis University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author M.T. Masucci M.T. Masucci University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author N. Behrendt N. Behrendt University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author L.R. Lund L.R. Lund University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author K. Danø K. Danø University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author E. Appella E. Appella University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author F. Blasi F. Blasi University Institute of Microbiology, Copenhagen, Denmark. Search for more papers by this author Author Information A.L. Roldan1, M.V. Cubellis1, M.T. Masucci1, N. Behrendt1, L.R. Lund1, K. Danø1, E. Appella1 and F. Blasi1 1University Institute of Microbiology, Copenhagen, Denmark. The EMBO Journal (1990)9:467-474https://doi.org/10.1002/j.1460-2075.1990.tb08132.x Correction(s) for this article Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysis01 May 1990 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The surface receptor for urokinase plasminogen activator (uPAR) has been recognized in recent years as a key molecule in regulating plasminogen mediated extracellular proteolysis. Surface plasminogen activation controls the connections between cells, basement membrane and extracellular matrix, and therefore the capacity of cells to migrate and invade neighboring tissues. We have isolated a 1.4 kb cDNA clone coding for the entire human uPAR. An oligonucleotide synthesized on the basis of the N-terminal sequence of the purified protein was used to screen a cDNA library made from SV40 transformed human fibroblasts [Okayama and Berg (1983) Mol. Cell Biol., 3, 280-289]. The cDNA encodes a protein of 313 amino acids, preceded by a 21 residue signal peptide. A hydrophobicity plot suggests the presence of a membrane spanning domain close to the C-terminus. The cDNA hybridizes to a 1.4 kb mRNA from human cells, a size very close to that of the cloned cDNA. Expression of the uPAR cDNA in mouse cells confirms that the clone is complete and expresses a functional uPA binding protein, located on the cell surface and with properties similar to the human uPAR. Caseinolytic plaque assay, immunofluorescence analysis, direct binding studies and cross-linking experiments show that the transfected mouse LB6 cells specifically bind human uPA, which in turn activates plasminogen. The Mr of the mature human receptor expressed in mouse cells is approximately 55,000, in accordance with the naturally occurring, highly glycosylated human uPAR. The Mr calculated on the basis of the cDNA sequence, approximately 35,000, agrees well with that of the deglycosylated receptor. Previous ArticleNext Article Volume 9Issue 21 February 1990In this issue RelatedDetailsLoading ..." @default.
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• W1853643611 title "Cloning and expression of the receptor for human urokinase plasminogen activator, a central molecule in cell surface, plasmin dependent proteolysis." @default.
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