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- W1854054867 abstract "Fluorescence-assisted mismatch analysis (FAMA) methodology uses bifluorescent double-stranded DNA substrates to maximize the reliability and sensitivity of mutation-scanning procedures that rely on cleavage of mismatches using chemical. This unit presents a nested PCR procedure to fluorescently label each DNA strand, followed by chemical cleavage to detect the point mutations. Fluorescent end labeling with strand-specific fluorophores, electrophoretic separation of cleavage products in an automated Perkin-Elmer ABI 373 or 377 DNA sequencer and analysis using the Perkin-Elmer GENESCAN software expands sensitivity by highlighting weak signals through superimposition of strand-specific fluorescence electropherograms for different samples." @default.
- W1854054867 created "2016-06-24" @default.
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- W1854054867 date "1997-02-01" @default.
- W1854054867 modified "2023-10-14" @default.
- W1854054867 title "Detection of Mutations by Fluorescence‐Assisted Mismatch Analysis (FAMA)" @default.
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- W1854054867 doi "https://doi.org/10.1002/0471142905.hg0708s12" @default.
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