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• W1854121465 endingPage "984" @default.
• W1854121465 startingPage "975" @default.
• W1854121465 abstract "We report on biocompatible, electrically conductive layers of polypyrrole (PPy)-based composites obtained by Matrix Assisted Pulsed Laser Evaporation (MAPLE) for envisioned bone regeneration. In order to preserve the conductivity of the PPy while overcoming its lack of biodegradability and low mechanical resilience, conductive PPy nanograins were embedded in two biocompatible, insulating polymeric matrices, i.e. poly(lactic-co-glycolic)acid (PLGA) and polyurethane (PU). PLGA offers the advantage of full biodegradability into non-toxic products, while PU provides toughness and elasticity. The PPy nanograins formed micro-domains and networks within the PLGA and PU matrices, in a compact spatial arrangement favorable for electrical percolation. The proposed approach allowed us to obtain PPy-based composite layers with biologically meaningful conductivities up to 10−2 S/cm for PPy loadings as low as 1:10 weight ratios. Fluorescent staining and viability assays showed that the MG63 osteoblast-like cells cultured on the PPy-based layers deposited by MAPLE were viable and retained their capacity to proliferate. The performance of the proposed method was demonstrated by quantitative evaluation of the calcium phosphate deposits from the cultured cells, as indicative for cell mineralization. Electrical stimulation using 200 μA currents passing through the PPy-based layers, during a time interval of 4 h, enhanced the osteogenesis in the cultured cells. Despite their lowest conductivity, the PPy/PU layers showed the best biocompatibility and the highest osteogenic potential." @default.
• W1854121465 created "2016-06-24" @default.
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• W1854121465 date "2015-12-01" @default.
• W1854121465 modified "2023-10-16" @default.
• W1854121465 title "MAPLE deposition of polypyrrole-based composite layers for bone regeneration" @default.
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• W1854121465 doi "https://doi.org/10.1016/j.apsusc.2015.09.083" @default.
• W1854121465 hasPublicationYear "2015" @default.
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