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- W18545478 abstract "Publisher Summary There are many situations in which it may be required to recover proteins from acrylamide gels for further analysis. For example, polypeptides extracted from gels can be readily used to immunize rabbits or mice to prepare antibodies. This chapter discusses a simple and effective method for electroelution of proteins from fixed, unstained, and Coomassie brilliant blue-stained dry two-dimensional (2D) gels. The method uses a silver-stained gel of electroeluted glutathione S-transferase π cut from a dry Coomassie brilliant blue-stained 2D gel of SV40-transformed keratinocytes. The sample was co-run with a small amount of 35 S-methionine-labeled proteins from the same cell type. However, the solution in the buffer chamber should be stirred to prevent bubbles from sticking to the bottom of the dialysis membrane. Sometimes, electroeluted proteins give rise to artifactual variants." @default.
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- W18545478 date "1994-01-01" @default.
- W18545478 modified "2023-10-15" @default.
- W18545478 title "Electroelution of Proteins from Two-Dimensional Gels" @default.
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- W18545478 doi "https://doi.org/10.1016/b978-0-12-164717-9.50039-8" @default.
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