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- W1854729004 abstract "Nucleic acids are the fundamental genetic material of all living organisms. With regard to the complex chemical composition of the macromolecule (nitrogenous bases and the sugar-phosphate chain, giving the polymer a negative charge at neutral pH) and widely varied arrangements of individual types of nucleic acids (native and singlestranded DNA, circular and supercoiled DNA, viral and phage RNAs, rRNA, mRNA, tRNA, DNA–RNA hybrids, natural and synthetic mono- and polythematic polymers, etc.), ionexchange, adsorption and partition chromatographic techniques and gel permeation chromatography can be used to separate nucleic acids. Of greater importance for tRNA are ion-exchange procedures on substituted celluloses and Sephadex, which permit the isolation of pure specific tRNAs that can be used for sequential analysis and for protein biosynthesis. The existence of weak bonding forces (secondary interactions dependent on RNA base composition) is of decisive importance in fractionation owing to the similarity of the separated molecules in terms of size and charge." @default.
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- W1854729004 date "1966-05-01" @default.
- W1854729004 modified "2023-10-11" @default.
- W1854729004 title "Extent and significance of contamination of DNA by teichoic acid in Bacillussubtilis" @default.
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- W1854729004 doi "https://doi.org/10.1016/0006-291x(66)90755-8" @default.
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