Matches in SemOpenAlex for { <https://semopenalex.org/work/W1854770036> ?p ?o ?g. }
- W1854770036 endingPage "4810" @default.
- W1854770036 startingPage "4797" @default.
- W1854770036 abstract "We have previously shown that the herpes simplex virus immediate-early regulatory protein ICP27 acts posttranscriptionally to affect mRNA processing (R. M. Sandri-Goldin and G. E. Mendoza, Genes Dev. 6:848-863, 1992). Specifically, in the presence of ICP27, spliced target mRNAs were decreased 5- to 10-fold in transfections with target genes containing a 5' or 3' intron. Here, we have investigated the effect of ICP27 during herpes simplex virus type 1 (HSV-1) infection on accumulation of spliced cellular mRNAs. ICP27 viral mutants have been shown to be defective in host shutoff (W. R. Sacks, C. C. Greene, D. P. Aschman, and P. A. Schaffer, J. Virol. 55:796-805, 1985). Therefore, we examined whether ICP27 could contribute to this complex process by decreasing cellular mRNA levels through its effects on host cell splicing. It was found that in infections with viral mutants defective in ICP27, the accumulated levels of three spliced host mRNAs were higher than those seen with wild-type HSV-1. The differences occurred posttranscriptionally as shown by nuclear runoff transcription assays. The stabilities of the spliced products during infection with wild-type or ICP27 mutant viruses were similar, and unspliced precursor mRNA for a viral spliced gene was detected in infections with wild-type HSV-1 but not in infections in which ICP27 was not expressed. These results suggest that the reduction in cellular mRNA levels and the accumulation of pre-mRNA are related and may be caused by an impairment in host cell splicing. These data further show that ICP27 is required for these effects to occur." @default.
- W1854770036 created "2016-06-24" @default.
- W1854770036 creator A5002805151 @default.
- W1854770036 creator A5087442186 @default.
- W1854770036 date "1994-08-01" @default.
- W1854770036 modified "2023-09-24" @default.
- W1854770036 title "The herpes simplex virus regulatory protein ICP27 contributes to the decrease in cellular mRNA levels during infection" @default.
- W1854770036 cites W1484990013 @default.
- W1854770036 cites W1509026992 @default.
- W1854770036 cites W1559441251 @default.
- W1854770036 cites W1579508447 @default.
- W1854770036 cites W1583466448 @default.
- W1854770036 cites W1584306191 @default.
- W1854770036 cites W1606830172 @default.
- W1854770036 cites W1611012144 @default.
- W1854770036 cites W1613555203 @default.
- W1854770036 cites W1614944940 @default.
- W1854770036 cites W1628312488 @default.
- W1854770036 cites W1635907978 @default.
- W1854770036 cites W1710344093 @default.
- W1854770036 cites W1733825645 @default.
- W1854770036 cites W1768938879 @default.
- W1854770036 cites W1799661214 @default.
- W1854770036 cites W1807366979 @default.
- W1854770036 cites W1834346265 @default.
- W1854770036 cites W1895304651 @default.
- W1854770036 cites W1898229461 @default.
- W1854770036 cites W1900646268 @default.
- W1854770036 cites W1938377731 @default.
- W1854770036 cites W1940400153 @default.
- W1854770036 cites W1964340993 @default.
- W1854770036 cites W1974534167 @default.
- W1854770036 cites W1978202804 @default.
- W1854770036 cites W1979281102 @default.
- W1854770036 cites W1981599663 @default.
- W1854770036 cites W1984734782 @default.
- W1854770036 cites W1989119084 @default.
- W1854770036 cites W2006558753 @default.
- W1854770036 cites W2013114542 @default.
- W1854770036 cites W2026073476 @default.
- W1854770036 cites W2029650577 @default.
- W1854770036 cites W2040114529 @default.
- W1854770036 cites W2041762780 @default.
- W1854770036 cites W2046261180 @default.
- W1854770036 cites W2047079821 @default.
- W1854770036 cites W2048167369 @default.
- W1854770036 cites W2056650797 @default.
- W1854770036 cites W2057328575 @default.
- W1854770036 cites W2077077811 @default.
- W1854770036 cites W2077262744 @default.
- W1854770036 cites W2081710371 @default.
- W1854770036 cites W2088986997 @default.
- W1854770036 cites W2091955128 @default.
- W1854770036 cites W2095142181 @default.
- W1854770036 cites W2105568629 @default.
- W1854770036 cites W2110277363 @default.
- W1854770036 cites W2118300475 @default.
- W1854770036 cites W2121542842 @default.
- W1854770036 cites W2125207784 @default.
- W1854770036 cites W2135921792 @default.
- W1854770036 cites W2144206181 @default.
- W1854770036 cites W2148406425 @default.
- W1854770036 cites W2164758952 @default.
- W1854770036 cites W2166195788 @default.
- W1854770036 cites W2168494912 @default.
- W1854770036 doi "https://doi.org/10.1128/jvi.68.8.4797-4810.1994" @default.
- W1854770036 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/236419" @default.
- W1854770036 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8035480" @default.
- W1854770036 hasPublicationYear "1994" @default.
- W1854770036 type Work @default.
- W1854770036 sameAs 1854770036 @default.
- W1854770036 citedByCount "178" @default.
- W1854770036 countsByYear W18547700362012 @default.
- W1854770036 countsByYear W18547700362013 @default.
- W1854770036 countsByYear W18547700362014 @default.
- W1854770036 countsByYear W18547700362015 @default.
- W1854770036 countsByYear W18547700362016 @default.
- W1854770036 countsByYear W18547700362017 @default.
- W1854770036 countsByYear W18547700362018 @default.
- W1854770036 countsByYear W18547700362019 @default.
- W1854770036 countsByYear W18547700362020 @default.
- W1854770036 countsByYear W18547700362021 @default.
- W1854770036 crossrefType "journal-article" @default.
- W1854770036 hasAuthorship W1854770036A5002805151 @default.
- W1854770036 hasAuthorship W1854770036A5087442186 @default.
- W1854770036 hasBestOaLocation W18547700361 @default.
- W1854770036 hasConcept C104317684 @default.
- W1854770036 hasConcept C105580179 @default.
- W1854770036 hasConcept C138885662 @default.
- W1854770036 hasConcept C143065580 @default.
- W1854770036 hasConcept C150194340 @default.
- W1854770036 hasConcept C153911025 @default.
- W1854770036 hasConcept C159047783 @default.
- W1854770036 hasConcept C179926584 @default.
- W1854770036 hasConcept C207583985 @default.
- W1854770036 hasConcept C2522874641 @default.
- W1854770036 hasConcept C2781196997 @default.
- W1854770036 hasConcept C41895202 @default.