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• W1856707908 startingPage "1263" @default.
• W1856707908 abstract "The chemical structure and biological properties of the lipopolysaccharide (LPS) from Vibrio cholerae 395 (Ogawa), isolated by the phenol-water procedure, were studied. Upon acid hydrolysis, the LPS was split into its polysaccharide and lipid A moieties. The polysaccharide contained both neutral (glucose, heptose, fructose) and amino (glucosamine, quinovosamine) sugars. The LPS contained the acid-labile amino sugar, 4-amino-arabinose, which was absent in the Inaba serotype of V. cholerae. The LPS differed from the LPSs of Enterobacteriaceae by the absence of 2-keto-3-deoxyoctonate and the presence of fructose. Analysis of the methylated polysaccharide by gas-liquid chromatography and mass spectrometry showed that it had a branched structure with glucose and heptose residues primarily appearing at the nonreducing-end groups. Interactions with lectins, concanavalin A. and wheat germ agglutinin suggested that terminal glucose residues were alpha linked, whereas terminal glucosamine residues were connected by alpha-1,3 linkages. The major fatty acids of the LPS were C14:0, C16:0, C12h:0, and C14h:0 compounds, of which only the C14h:0 were amide linked, the remainder being ester linked to the backbone. Biological studies showed that the LPS possessed endotoxic properties such as lethality, pyrogenicity, limulus lysate gelation, and ability to induce non-specific resistance to infection. Thus, the LPS from V. cholerae 395 (Ogawa) possessed both common and distinct features as compared with the LPSs from the Enterobacteriaceae." @default.
• W1856707908 created "2016-06-24" @default.
• W1856707908 creator A5080592861 @default.
• W1856707908 date "1982-12-01" @default.
• W1856707908 modified "2023-09-26" @default.
• W1856707908 title "Characterization of the lipopolysaccharide from Vibrio cholerae 395 (Ogawa)" @default.
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• W1856707908 doi "https://doi.org/10.1128/iai.38.3.1263-1272.1982" @default.
• W1856707908 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/347884" @default.
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• W1856707908 hasPublicationYear "1982" @default.
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