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- W18568254 abstract "Estradiol (E2) is present in milk, but the mechanism(s) regulating the appearance of E2 in milk are not known. The objectives of this study were to quantify E2 concentrations in plasma and milk samples throughout pregnancy in cows and to determine if the plasma to milk ratio changes during pregnancy. Blood and milk samples were collected from 13 cows every 28 days throughout pregnancy. E2 was quantified in blood and milk using radioimmunoassay after organic solvent extractions and Sephadex LH-20 column chromatography. Cows were categorized in the 1st, 2nd, and 3rd trimesters of pregnancy. Proc Mixed and Corr procedures of the Statistical Analysis Software were utilized for statistical analyses. Plasma E2 averaged 0.55 ± 0.22, 0.86 ± 0.17, and 2.01 ± 0.20 pg/mL and milk E2 averaged 0.25 ± 0.90, 0.87 ± 0.67, and 5.09 ± 0.61 pg/mL for trimesters 1, 2, and 3, respectively. Plasma and milk E2 concentrations were significantly higher in trimester 3 compared to 1 and 2 (P < 0.001). Milk E2 concentrations were positively correlated with milk fat (R = 0.22, P < 0.05), milk protein (R = 0.62, P < 0.001) and plasma E2 concentrations (R = 0.86, P < 0.001). The plasma to milk ratio of E2 significantly decreased with ratios of 3.0, 1.7, and 0.6 in trimesters 1, 2, and 3, respectively, suggesting that the mechanism(s) regulating the appearance of E2 in milk may change during late pregnancy. (USDA Special Research Grant 2003-34163-13404)." @default.
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- W18568254 date "2007-01-01" @default.
- W18568254 modified "2023-09-26" @default.
- W18568254 title "Estradiol concentrations increase in bovine milk in late pregnancy and are correlated with milk fat and protein" @default.
- W18568254 doi "https://doi.org/10.1096/fasebj.21.6.a1423-d" @default.
- W18568254 hasPublicationYear "2007" @default.
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