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• W1857180815 endingPage "26372" @default.
• W1857180815 startingPage "26361" @default.
• W1857180815 abstract "Triacylglycerols (TGs) stored in lipid droplets (LDs) are hydrolyzed in a highly regulated metabolic process called lipolysis to free fatty acids that serve as energy substrates for β-oxidation, precursors for membrane lipids and signaling molecules. Comparative gene identification-58 (CGI-58) stimulates the enzymatic activity of adipose triglyceride lipase (ATGL), which catalyzes the hydrolysis of TGs to diacylglycerols and free fatty acids. In adipose tissue, protein-protein interactions between CGI-58 and the LD coating protein perilipin 1 restrain the ability of CGI-58 to activate ATGL under basal conditions. Phosphorylation of perilipin 1 disrupts these interactions and mobilizes CGI-58 for the activation of ATGL. We have previously demonstrated that the removal of a peptide at the N terminus (residues 10–31) of CGI-58 abrogates CGI-58 localization to LDs and CGI-58-mediated activation of ATGL. Here, we show that this tryptophan-rich N-terminal peptide serves as an independent LD anchor, with its three tryptophans serving as focal points of the left (harboring Trp21 and Trp25) and right (harboring Trp29) anchor arms. The solution state NMR structure of a peptide comprising the LD anchor bound to dodecylphosphocholine micelles as LD mimic reveals that the left arm forms a concise hydrophobic core comprising tryptophans Trp21 and Trp25 and two adjacent leucines. Trp29 serves as the core of a functionally independent anchor arm. Consequently, simultaneous tryptophan alanine permutations in both arms abolish localization and activity of CGI-58 as opposed to tryptophan substitutions that occur in only one arm. Triacylglycerols (TGs) stored in lipid droplets (LDs) are hydrolyzed in a highly regulated metabolic process called lipolysis to free fatty acids that serve as energy substrates for β-oxidation, precursors for membrane lipids and signaling molecules. Comparative gene identification-58 (CGI-58) stimulates the enzymatic activity of adipose triglyceride lipase (ATGL), which catalyzes the hydrolysis of TGs to diacylglycerols and free fatty acids. In adipose tissue, protein-protein interactions between CGI-58 and the LD coating protein perilipin 1 restrain the ability of CGI-58 to activate ATGL under basal conditions. Phosphorylation of perilipin 1 disrupts these interactions and mobilizes CGI-58 for the activation of ATGL. We have previously demonstrated that the removal of a peptide at the N terminus (residues 10–31) of CGI-58 abrogates CGI-58 localization to LDs and CGI-58-mediated activation of ATGL. Here, we show that this tryptophan-rich N-terminal peptide serves as an independent LD anchor, with its three tryptophans serving as focal points of the left (harboring Trp21 and Trp25) and right (harboring Trp29) anchor arms. The solution state NMR structure of a peptide comprising the LD anchor bound to dodecylphosphocholine micelles as LD mimic reveals that the left arm forms a concise hydrophobic core comprising tryptophans Trp21 and Trp25 and two adjacent leucines. Trp29 serves as the core of a functionally independent anchor arm. Consequently, simultaneous tryptophan alanine permutations in both arms abolish localization and activity of CGI-58 as opposed to tryptophan substitutions that occur in only one arm." @default.
• W1857180815 created "2016-06-24" @default.
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• W1857180815 date "2015-10-01" @default.
• W1857180815 modified "2023-10-15" @default.
• W1857180815 title "Structure of a CGI-58 Motif Provides the Molecular Basis of Lipid Droplet Anchoring" @default.
• W1857180815 cites W1188919047 @default.
• W1857180815 cites W1563233557 @default.
• W1857180815 cites W1880173361 @default.
• W1857180815 cites W1964376459 @default.
• W1857180815 cites W1968712781 @default.
• W1857180815 cites W1969624192 @default.
• W1857180815 cites W1975459052 @default.
• W1857180815 cites W1978921042 @default.
• W1857180815 cites W1982560816 @default.
• W1857180815 cites W1986318660 @default.
• W1857180815 cites W1986413623 @default.
• W1857180815 cites W1991285104 @default.
• W1857180815 cites W1994146146 @default.
• W1857180815 cites W2004377868 @default.
• W1857180815 cites W2008636063 @default.
• W1857180815 cites W2010819467 @default.
• W1857180815 cites W2014100153 @default.
• W1857180815 cites W2014154105 @default.
• W1857180815 cites W2015437105 @default.
• W1857180815 cites W2015898031 @default.
• W1857180815 cites W2016078643 @default.
• W1857180815 cites W2017466835 @default.
• W1857180815 cites W2020389453 @default.
• W1857180815 cites W2021224212 @default.
• W1857180815 cites W2023430066 @default.
• W1857180815 cites W2023430604 @default.
• W1857180815 cites W2024755768 @default.
• W1857180815 cites W2029318573 @default.
• W1857180815 cites W2036928394 @default.
• W1857180815 cites W2042837261 @default.
• W1857180815 cites W2044073767 @default.
• W1857180815 cites W2046282779 @default.
• W1857180815 cites W2048606831 @default.
• W1857180815 cites W2051548651 @default.
• W1857180815 cites W2052489013 @default.
• W1857180815 cites W2052933818 @default.
• W1857180815 cites W2058668243 @default.
• W1857180815 cites W2059069942 @default.
• W1857180815 cites W2059877647 @default.
• W1857180815 cites W2060178110 @default.
• W1857180815 cites W2061795728 @default.
• W1857180815 cites W2069051641 @default.
• W1857180815 cites W2071608742 @default.
• W1857180815 cites W2075386236 @default.
• W1857180815 cites W2081435829 @default.
• W1857180815 cites W2085578279 @default.
• W1857180815 cites W2085672416 @default.
• W1857180815 cites W2086862822 @default.
• W1857180815 cites W2088848851 @default.
• W1857180815 cites W2090946848 @default.
• W1857180815 cites W2093596830 @default.
• W1857180815 cites W2099698581 @default.
• W1857180815 cites W2101543956 @default.
• W1857180815 cites W2101675947 @default.
• W1857180815 cites W2106442657 @default.
• W1857180815 cites W2110079579 @default.
• W1857180815 cites W2110839026 @default.
• W1857180815 cites W2111219397 @default.
• W1857180815 cites W2112418305 @default.
• W1857180815 cites W2121233961 @default.
• W1857180815 cites W2125355462 @default.
• W1857180815 cites W2129406204 @default.
• W1857180815 cites W2129545557 @default.
• W1857180815 cites W2129744834 @default.
• W1857180815 cites W2141582150 @default.
• W1857180815 cites W2144231371 @default.
• W1857180815 cites W2147467792 @default.
• W1857180815 cites W2148521301 @default.
• W1857180815 cites W2157817407 @default.
• W1857180815 cites W2169707043 @default.
• W1857180815 cites W2169821755 @default.
• W1857180815 cites W2171254281 @default.
• W1857180815 cites W2172191429 @default.
• W1857180815 cites W4229743141 @default.
• W1857180815 doi "https://doi.org/10.1074/jbc.m115.682203" @default.
• W1857180815 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4646293" @default.
• W1857180815 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26350461" @default.
• W1857180815 hasPublicationYear "2015" @default.
• W1857180815 type Work @default.
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