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• W1857373397 startingPage "3407" @default.
• W1857373397 abstract "The direct repeat (DR) sequences flanking the src gene in Rous sarcoma virus are essential posttranscriptional control elements; at least one copy of this sequence is necessary for cytoplasmic accumulation of unspliced viral RNA. These sequences promote Rev-independent human immunodeficiency virus type 1 expression, suggesting they act as constitutive transport elements (CTEs). To determine which regions of this sequence are critical for CTE function, mutations in the downstream DR were generated and tested in a viral deletion construct lacking src and the upstream DR. Two single-point mutations and three different clustered mutations caused substantial reductions in reverse transcriptase activity, Gag protein levels, and unspliced viral RNA in the cytoplasm. Three conserved regions of the CTE, including nucleotides 8844 to 8847, 8862 to 8864, and 8868 to 8870, were most sensitive to inactivation by mutagenesis." @default.
• W1857373397 created "2016-06-24" @default.
• W1857373397 creator A5046483556 @default.
• W1857373397 creator A5061833373 @default.
• W1857373397 date "1998-04-01" @default.
• W1857373397 modified "2023-09-25" @default.
• W1857373397 title "Mutational Analysis of the Rous Sarcoma Virus DR Posttranscriptional Control Element" @default.
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• W1857373397 doi "https://doi.org/10.1128/jvi.72.4.3407-3411.1998" @default.
• W1857373397 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/109836" @default.
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