FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1858059246> ?p ?o ?g. }

• W1858059246 endingPage "2353" @default.
• W1858059246 startingPage "2341" @default.
• W1858059246 abstract "Attaching and effacing Escherichia coli (AEEC) strains isolated from diarrhoeic lambs and goat kids were characterized for intimin (eae) and EspB (espB) gene subtypes by PCR and sequencing, and for genetic relatedness by PFGE. Fifty (23 ovine and 27 caprine) AEEC strains of 398 (246 ovine and 152 caprine) analysed were detected by colony blot hybridization. These strains were epidemiologically unrelated since they were isolated from different outbreaks of neonatal diarrhoea over a long period. Ovine AEEC strains belonged to serogroups O2, O4, O26, O80, O91 or were untypable, and caprine strains belonged to serogroups O3, O153 and O163. Two intimin subtypes were detected among the ovine and caprine strains studied. Most of the strains (43/50) had the beta type intimin gene, but seven ovine strains possessed a variant gamma type intimin gene (gamma(V)). Analysis of deduced amino acid sequences of the eae gene revealed that the sequences of beta intimin of ovine and caprine strains were virtually identical to those of beta intimin of rabbit EPEC, human EPEC clone 2 and swine AEEC, whereas the gamma(V) intimin present in seven ovine strains had 75-76% identity with gamma intimin of human EHEC clone 1 strains, and 96% of identity with intimin of the human EHEC strain 95NR1 of serotype O111:H-. A PCR test was developed to identify the three different espB gene subtypes, espB of human EPEC clone 1 (espBalpha), espB of human EHEC clone 1 (espBgamma) and espB of rabbit EPEC and human EPEC clone 2 (espBbeta). There was close correlation between the intimin beta type and the espBbeta gene subtype in the ovine and caprine AEEC strains. The seven ovine strains possessing the gamma(V) intimin gene possessed the espBalpha gene subtype. None of the strains studied possessed the espBgamma gene found in human O157:H7 EHEC strains. PFGE analysis of genomic DNA of selected strains showed a great diversity among strains. Cluster analysis of PFGE patterns showed greater divergence between strains with the gamma(V) intimin gene than between strains with the beta intimin gene. This study showed that most of the AEEC strains isolated from diarrhoeic lambs and goat kids possessed beta intimin and espB genes identical to those of rabbit EPEC, and they may be associated with enteric disease in small ruminants." @default.
• W1858059246 created "2016-06-24" @default.
• W1858059246 creator A5000994199 @default.
• W1858059246 creator A5005185558 @default.
• W1858059246 creator A5012725123 @default.
• W1858059246 creator A5034597750 @default.
• W1858059246 creator A5042440604 @default.
• W1858059246 creator A5046473514 @default.
• W1858059246 creator A5090916748 @default.
• W1858059246 date "2001-08-01" @default.
• W1858059246 modified "2023-10-02" @default.
• W1858059246 title "Association between intimin (eae) and EspB gene subtypes in attaching and effacing Escherichia coli strains isolated from diarrhoeic lambs and goat kids The GenBank accession numbers for the sequences reported in this paper are AF253560 (eae gene of strain CK379), AF253561 (eae gene of strain CL559), and AF254454, AF254455, AF254456 and AF254457 (espB nucleotide sequences of strains CL559, CL617, CK379 and CL398, respectively)" @default.
• W1858059246 cites W1492600174 @default.
• W1858059246 cites W1582464264 @default.
• W1858059246 cites W1583084344 @default.
• W1858059246 cites W1622915373 @default.
• W1858059246 cites W1814968550 @default.
• W1858059246 cites W1833131008 @default.
• W1858059246 cites W1904815041 @default.
• W1858059246 cites W1914282190 @default.
• W1858059246 cites W1915744985 @default.
• W1858059246 cites W1955612312 @default.
• W1858059246 cites W1970851377 @default.
• W1858059246 cites W1975057095 @default.
• W1858059246 cites W2002543993 @default.
• W1858059246 cites W2006321114 @default.
• W1858059246 cites W2024083251 @default.
• W1858059246 cites W2034564105 @default.
• W1858059246 cites W2045318756 @default.
• W1858059246 cites W2051347791 @default.
• W1858059246 cites W2055120082 @default.
• W1858059246 cites W2061060437 @default.
• W1858059246 cites W2068760242 @default.
• W1858059246 cites W2069158367 @default.
• W1858059246 cites W2075135803 @default.
• W1858059246 cites W2075151436 @default.
• W1858059246 cites W2094031081 @default.
• W1858059246 cites W2097300280 @default.
• W1858059246 cites W2102313682 @default.
• W1858059246 cites W2110641481 @default.
• W1858059246 cites W2112111992 @default.
• W1858059246 cites W2112216397 @default.
• W1858059246 cites W2118585691 @default.
• W1858059246 cites W2119453756 @default.
• W1858059246 cites W2124374848 @default.
• W1858059246 cites W2127912613 @default.
• W1858059246 cites W2133073740 @default.
• W1858059246 cites W2135894069 @default.
• W1858059246 cites W2138851755 @default.
• W1858059246 cites W2145121851 @default.
• W1858059246 cites W2152942945 @default.
• W1858059246 cites W2159227713 @default.
• W1858059246 cites W2161186278 @default.
• W1858059246 cites W2163491119 @default.
• W1858059246 cites W2167181980 @default.
• W1858059246 cites W2170276300 @default.
• W1858059246 cites W4244170547 @default.
• W1858059246 doi "https://doi.org/10.1099/00221287-147-8-2341" @default.
• W1858059246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11496011" @default.
• W1858059246 hasPublicationYear "2001" @default.
• W1858059246 type Work @default.
• W1858059246 sameAs 1858059246 @default.
• W1858059246 citedByCount "56" @default.
• W1858059246 countsByYear W18580592462012 @default.
• W1858059246 countsByYear W18580592462013 @default.
• W1858059246 countsByYear W18580592462014 @default.
• W1858059246 countsByYear W18580592462015 @default.
• W1858059246 countsByYear W18580592462016 @default.
• W1858059246 countsByYear W18580592462017 @default.
• W1858059246 countsByYear W18580592462018 @default.
• W1858059246 countsByYear W18580592462019 @default.
• W1858059246 countsByYear W18580592462021 @default.
• W1858059246 countsByYear W18580592462022 @default.
• W1858059246 countsByYear W18580592462023 @default.
• W1858059246 crossrefType "journal-article" @default.
• W1858059246 hasAuthorship W1858059246A5000994199 @default.
• W1858059246 hasAuthorship W1858059246A5005185558 @default.
• W1858059246 hasAuthorship W1858059246A5012725123 @default.
• W1858059246 hasAuthorship W1858059246A5034597750 @default.
• W1858059246 hasAuthorship W1858059246A5042440604 @default.
• W1858059246 hasAuthorship W1858059246A5046473514 @default.
• W1858059246 hasAuthorship W1858059246A5090916748 @default.
• W1858059246 hasBestOaLocation W18580592461 @default.
• W1858059246 hasConcept C10389963 @default.
• W1858059246 hasConcept C104317684 @default.
• W1858059246 hasConcept C118815799 @default.
• W1858059246 hasConcept C159047783 @default.
• W1858059246 hasConcept C2778910516 @default.
• W1858059246 hasConcept C54355233 @default.
• W1858059246 hasConcept C547475151 @default.
• W1858059246 hasConcept C81089528 @default.
• W1858059246 hasConcept C86803240 @default.
• W1858059246 hasConcept C89423630 @default.
• W1858059246 hasConceptScore W1858059246C10389963 @default.
• W1858059246 hasConceptScore W1858059246C104317684 @default.
• W1858059246 hasConceptScore W1858059246C118815799 @default.
• W1858059246 hasConceptScore W1858059246C159047783 @default.
• W1858059246 hasConceptScore W1858059246C2778910516 @default.

• next