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• W1858455433 abstract "The limited healing capacity of articular cartilage forms a major clinical problem. In general, current treatments of cartilage damage temporarily reliefs symptoms, but fail in the long term. Tissue engineering (TE) has been proposed as a more permanent repair strategy. Cartilage TE aims at cultivating suitable implant material for cartilage repair. This has to be achieved by providing a favorable environment for cartilage regeneration in vitro (Chapter 1). The following phases can be distinguished: First, cells are isolated from the patient and expanded in culture to obtain sufficient numbers. Then the cells are seeded in a scaffold, i.e. a biodegradable three-dimensional structure that provides support for the cells. Subsequently, during culture in a bioreactor, the cells are conditioned to synthesize extracellular matrix components, resulting in neo-cartilage formation. Eventually, the newly formed cartilage construct has to acquire adequate mechanical properties, in order to be reimplanted into the patient. However, in general the mechanical properties of TE cartilage do not meet these functional requirements. In addition there is a definite lack of control over the functional development of tissue engineered cartilage. Current research is mainly characterized by a strong experimental basis. The use of computational methods is relatively limited, while modeling can potentially provide an important contribution to the optimization of bioreactors and culture protocols. In addition, modeling can prove useful in the reduction of the number of experiments and the interpretation of the results. In the present thesis, a computational approach is proposed that enables the modeling of the functional development of tissue engineered cartilage. The computational method is applied to establish relationships between mechanical stimulation of cartilage constructs by dynamic compression and transport of solutes, for example nutrients, within these constructs (Chapter 2). It is shown that compression-induced fluid flow can affect the transport of large solutes but not that of small solutes. This has implications for matrix synthesis. A microstructural homogenization approach is used to determine how the synthesis of extracellular matrix at the cellular level, translates in the evolving mechanical properties of the newly formed cartilage as a whole (Chapter 3). The results indicate that the matrix distribution at the cellular level may be of less importance than its molecular organization. With respect to the kinetics of cartilage formation, it is known that cellular energy metabolism is closely associated with the synthesis of extracellular matrix. Therefore, using the model, the cellular uptake of glucose and oxygen and the production of lactate is characterized on the basis of experimental data (Chapter 4). It is shown that cellular uptake in the experiments was influenced by both the initial glucose concentration in the culture medium and the cell density in the construct. Matrix synthesis and proliferation can be stimulated in an uncoupled manner, by applying different time intervals of dynamic compression. In order to interpret and predict cell behavior, models are developed for the temporal regulation of chondrocyte proliferation and biosynthesis, in response to varying dynamic compression regimens (Chapter 5). These models are able to provide a reasonable overall representation of experimental results for different loading regimens. However, for specific loading cases the predictive value is limited. Subsequently, cellular uptake data is used to predict, numerically, the nutrient supply in different bioreactor setups and to evaluate the effect of mixing, perfusion and geometry (Chapter 6). The results indicate that transport limitations are not insurmountable, providing that the bioreactor environment is well homogenized and oxygenated. It can be concluded that the proposed modeling approach can provide a valuable contribution in determining optimal culture conditions for cartilage tissue engineering (Chapter 7). At the present stage, additional identification and quantification of chondrocyte behavior is necessary, with respect to utilization, biosynthesis and mechanotransduction. In addition, criteria with respect to matrix synthesis have to be established." @default.
• W1858455433 created "2016-06-24" @default.
• W1858455433 creator A5026365000 @default.
• W1858455433 date "2005-01-01" @default.
• W1858455433 modified "2023-09-23" @default.
• W1858455433 title "Modeling the development of tissue engineered cartilage" @default.
• W1858455433 cites W1515651002 @default.
• W1858455433 cites W154044508 @default.
• W1858455433 cites W1548480021 @default.
• W1858455433 cites W1591593323 @default.
• W1858455433 cites W1606720378 @default.
• W1858455433 cites W1614928787 @default.
• W1858455433 cites W1722420563 @default.
• W1858455433 cites W1825268336 @default.
• W1858455433 cites W1829465060 @default.
• W1858455433 cites W1873506857 @default.
• W1858455433 cites W1920660862 @default.
• W1858455433 cites W1938952532 @default.
• W1858455433 cites W1963013017 @default.
• W1858455433 cites W1964813629 @default.
• W1858455433 cites W1965878872 @default.
• W1858455433 cites W1966066347 @default.
• W1858455433 cites W1966291445 @default.
• W1858455433 cites W1968277140 @default.
• W1858455433 cites W1968609580 @default.
• W1858455433 cites W1969113747 @default.
• W1858455433 cites W1969622566 @default.
• W1858455433 cites W1970656189 @default.
• W1858455433 cites W1972349420 @default.
• W1858455433 cites W1973757034 @default.
• W1858455433 cites W1974949986 @default.
• W1858455433 cites W1975098157 @default.
• W1858455433 cites W1975593347 @default.
• W1858455433 cites W1977581444 @default.
• W1858455433 cites W1978619076 @default.
• W1858455433 cites W1979384058 @default.
• W1858455433 cites W1980440152 @default.
• W1858455433 cites W1982713034 @default.
• W1858455433 cites W1982938005 @default.
• W1858455433 cites W1983051682 @default.
• W1858455433 cites W1983570119 @default.
• W1858455433 cites W1985074026 @default.
• W1858455433 cites W1985670094 @default.
• W1858455433 cites W1989830594 @default.
• W1858455433 cites W1989892130 @default.
• W1858455433 cites W1990853549 @default.
• W1858455433 cites W1990859263 @default.
• W1858455433 cites W1990915139 @default.
• W1858455433 cites W1992193707 @default.
• W1858455433 cites W1994537168 @default.
• W1858455433 cites W1997299213 @default.
• W1858455433 cites W1997668164 @default.
• W1858455433 cites W2000571117 @default.
• W1858455433 cites W2002100798 @default.
• W1858455433 cites W2006038006 @default.
• W1858455433 cites W2008236596 @default.
• W1858455433 cites W2010902667 @default.
• W1858455433 cites W2013674234 @default.
• W1858455433 cites W2013682631 @default.
• W1858455433 cites W2014799804 @default.
• W1858455433 cites W2014849732 @default.
• W1858455433 cites W2015694728 @default.
• W1858455433 cites W2017358101 @default.
• W1858455433 cites W2018362210 @default.
• W1858455433 cites W2020342708 @default.
• W1858455433 cites W2020787517 @default.
• W1858455433 cites W2022020242 @default.
• W1858455433 cites W2023592124 @default.
• W1858455433 cites W2024934402 @default.
• W1858455433 cites W2026220322 @default.
• W1858455433 cites W2028596690 @default.
• W1858455433 cites W2029357468 @default.
• W1858455433 cites W2029621921 @default.
• W1858455433 cites W2031673412 @default.
• W1858455433 cites W2033783682 @default.
• W1858455433 cites W2034463010 @default.
• W1858455433 cites W2034607468 @default.
• W1858455433 cites W2035469813 @default.
• W1858455433 cites W2035528429 @default.
• W1858455433 cites W2036087914 @default.
• W1858455433 cites W2036646846 @default.
• W1858455433 cites W2037570346 @default.
• W1858455433 cites W2038650099 @default.
• W1858455433 cites W2038752002 @default.
• W1858455433 cites W2038993876 @default.
• W1858455433 cites W2041126017 @default.
• W1858455433 cites W2042517754 @default.
• W1858455433 cites W2045206656 @default.
• W1858455433 cites W2045443268 @default.
• W1858455433 cites W2046246025 @default.
• W1858455433 cites W2048276432 @default.
• W1858455433 cites W2048577098 @default.
• W1858455433 cites W2049024822 @default.
• W1858455433 cites W2049161806 @default.
• W1858455433 cites W2050624061 @default.
• W1858455433 cites W2051761181 @default.
• W1858455433 cites W2052968052 @default.
• W1858455433 cites W2055236428 @default.
• W1858455433 cites W2055884129 @default.
• W1858455433 cites W2055933712 @default.

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