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- W1858743365 abstract "The brain’s serotonergic system centrally regulates several physiological processes and its dysfunction has been implicated in the pathophysiology of several neuropsychiatric disorders. While in the past our understanding of serotonergic neurotransmission has come mainly from mouse models, the development of pluripotent stem cell and induced fibroblast-to-neuron (iN) transdifferentiation technologies has revolutionized our ability to generate human neurons in vitro. Utilizing these techniques and a novel lentiviral reporter for serotonergic neurons, we identified and overexpressed key transcription factors to successfully generate human serotonergic neurons. We found that overexpressing the transcription factors NKX2.2, FEV, GATA2 and LMX1B in combination with ASCL1 and NGN2 directly and efficiently generated serotonergic neurons from human fibroblasts. Induced serotonergic neurons (iSNs) showed increased expression of specific serotonergic genes that are known to be expressed in raphe nuclei. iSNs displayed spontaneous action potentials, released serotonin in vitro and functionally responded to selective serotonin reuptake inhibitors (SSRIs). Here, we demonstrate the efficient generation of functional human serotonergic neurons from human fibroblasts as a novel tool for studying human serotonergic neurotransmission in health and disease." @default.
- W1858743365 created "2016-06-24" @default.
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- W1858743365 date "2015-10-27" @default.
- W1858743365 modified "2023-10-15" @default.
- W1858743365 title "Generation of functional human serotonergic neurons from fibroblasts" @default.
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- W1858743365 cites W1973544245 @default.
- W1858743365 cites W1978257180 @default.
- W1858743365 cites W1979683490 @default.
- W1858743365 cites W1981437331 @default.
- W1858743365 cites W1983274440 @default.
- W1858743365 cites W1986926390 @default.
- W1858743365 cites W1987167495 @default.
- W1858743365 cites W1988394934 @default.
- W1858743365 cites W1995217110 @default.
- W1858743365 cites W1995610071 @default.
- W1858743365 cites W2002051646 @default.
- W1858743365 cites W2003835684 @default.
- W1858743365 cites W2005965747 @default.
- W1858743365 cites W2007644698 @default.
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- W1858743365 cites W2014444260 @default.
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- W1858743365 cites W2035645839 @default.
- W1858743365 cites W2041907941 @default.
- W1858743365 cites W2044347158 @default.
- W1858743365 cites W2045997356 @default.
- W1858743365 cites W2049665308 @default.
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- W1858743365 cites W2068165326 @default.
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- W1858743365 cites W2136472021 @default.
- W1858743365 cites W2136541538 @default.
- W1858743365 cites W2144217996 @default.
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- W1858743365 doi "https://doi.org/10.1038/mp.2015.161" @default.
- W1858743365 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26503761" @default.
- W1858743365 hasPublicationYear "2015" @default.
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