Matches in SemOpenAlex for { <https://semopenalex.org/work/W185891504> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W185891504 endingPage "373" @default.
- W185891504 startingPage "369" @default.
- W185891504 abstract "Simple, fast and cost-effective method for preparation of DNA with high molecular weight (HMW DNA) from plant nuclei and mitotic chromosomes has been developed. The technique involves mechanical homogenization of formaldehyde-fixed root tips, purification of nuclei and/or chromosomes on sucrose gradient, embedding in low-melting-point agarose, and DNA isolation in agarose plugs. Alternatively, nuclei and chromosomes may be purified using flow cytometry. Majority of DNA obtained is megabase-sized and well digestible by restriction endonucleases. The method is highly efficient as microgram amounts of DNA can be obtained from only several milligrams of plant tissue. Handling negligible amounts of plant material reduces the consumption of chemicals. Furthermore, the use of root tips makes it possible to obtain high-quality DNA even from plant species with leaves that are rigid or rich in secondary metabolites such as polyphenols. It is expected that preparation of HMW DNA from root tip nuclei will facilitate long-range mapping and construction of large-insert DNA libraries also in these species. Successful isolation of HMW DNA from flow-sorted chromosomes opens a way for construction of chromosome-specific large-insert libraries in plants." @default.
- W185891504 created "2016-06-24" @default.
- W185891504 creator A5009385040 @default.
- W185891504 creator A5043376977 @default.
- W185891504 creator A5044113178 @default.
- W185891504 creator A5063337128 @default.
- W185891504 creator A5071812121 @default.
- W185891504 date "2003-11-01" @default.
- W185891504 modified "2023-10-16" @default.
- W185891504 title "Preparation of HMW DNA from Plant Nuclei and Chromosomes Isolated from Root Tips" @default.
- W185891504 cites W1565467810 @default.
- W185891504 cites W193854521 @default.
- W185891504 cites W1992546101 @default.
- W185891504 cites W2042383707 @default.
- W185891504 cites W2054070623 @default.
- W185891504 cites W2066763890 @default.
- W185891504 cites W2073198696 @default.
- W185891504 cites W2073226443 @default.
- W185891504 cites W2090832876 @default.
- W185891504 cites W2091543204 @default.
- W185891504 cites W2091973256 @default.
- W185891504 cites W2892994881 @default.
- W185891504 doi "https://doi.org/10.1023/a:1024322001786" @default.
- W185891504 hasPublicationYear "2003" @default.
- W185891504 type Work @default.
- W185891504 sameAs 185891504 @default.
- W185891504 citedByCount "58" @default.
- W185891504 countsByYear W1858915042012 @default.
- W185891504 countsByYear W1858915042013 @default.
- W185891504 countsByYear W1858915042014 @default.
- W185891504 countsByYear W1858915042015 @default.
- W185891504 countsByYear W1858915042016 @default.
- W185891504 countsByYear W1858915042017 @default.
- W185891504 countsByYear W1858915042018 @default.
- W185891504 countsByYear W1858915042021 @default.
- W185891504 countsByYear W1858915042022 @default.
- W185891504 countsByYear W1858915042023 @default.
- W185891504 crossrefType "journal-article" @default.
- W185891504 hasAuthorship W185891504A5009385040 @default.
- W185891504 hasAuthorship W185891504A5043376977 @default.
- W185891504 hasAuthorship W185891504A5044113178 @default.
- W185891504 hasAuthorship W185891504A5063337128 @default.
- W185891504 hasAuthorship W185891504A5071812121 @default.
- W185891504 hasBestOaLocation W1858915041 @default.
- W185891504 hasConcept C104317684 @default.
- W185891504 hasConcept C130217890 @default.
- W185891504 hasConcept C153911025 @default.
- W185891504 hasConcept C181199279 @default.
- W185891504 hasConcept C18903297 @default.
- W185891504 hasConcept C2778476535 @default.
- W185891504 hasConcept C2778722038 @default.
- W185891504 hasConcept C2991965276 @default.
- W185891504 hasConcept C2992022053 @default.
- W185891504 hasConcept C30481170 @default.
- W185891504 hasConcept C49105822 @default.
- W185891504 hasConcept C552990157 @default.
- W185891504 hasConcept C55493867 @default.
- W185891504 hasConcept C59822182 @default.
- W185891504 hasConcept C78063203 @default.
- W185891504 hasConcept C86803240 @default.
- W185891504 hasConceptScore W185891504C104317684 @default.
- W185891504 hasConceptScore W185891504C130217890 @default.
- W185891504 hasConceptScore W185891504C153911025 @default.
- W185891504 hasConceptScore W185891504C181199279 @default.
- W185891504 hasConceptScore W185891504C18903297 @default.
- W185891504 hasConceptScore W185891504C2778476535 @default.
- W185891504 hasConceptScore W185891504C2778722038 @default.
- W185891504 hasConceptScore W185891504C2991965276 @default.
- W185891504 hasConceptScore W185891504C2992022053 @default.
- W185891504 hasConceptScore W185891504C30481170 @default.
- W185891504 hasConceptScore W185891504C49105822 @default.
- W185891504 hasConceptScore W185891504C552990157 @default.
- W185891504 hasConceptScore W185891504C55493867 @default.
- W185891504 hasConceptScore W185891504C59822182 @default.
- W185891504 hasConceptScore W185891504C78063203 @default.
- W185891504 hasConceptScore W185891504C86803240 @default.
- W185891504 hasIssue "3" @default.
- W185891504 hasLocation W1858915041 @default.
- W185891504 hasOpenAccess W185891504 @default.
- W185891504 hasPrimaryLocation W1858915041 @default.
- W185891504 hasRelatedWork W166351930 @default.
- W185891504 hasRelatedWork W185891504 @default.
- W185891504 hasRelatedWork W2025913943 @default.
- W185891504 hasRelatedWork W2033636902 @default.
- W185891504 hasRelatedWork W2104620528 @default.
- W185891504 hasRelatedWork W2312674383 @default.
- W185891504 hasRelatedWork W2394422079 @default.
- W185891504 hasRelatedWork W2416084300 @default.
- W185891504 hasRelatedWork W2757910772 @default.
- W185891504 hasRelatedWork W4249473811 @default.
- W185891504 hasVolume "46" @default.
- W185891504 isParatext "false" @default.
- W185891504 isRetracted "false" @default.
- W185891504 magId "185891504" @default.
- W185891504 workType "article" @default.