Matches in SemOpenAlex for { <https://semopenalex.org/work/W1859392330> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W1859392330 abstract "In earlier studies Na(+)-K(+)-adenosinetriphosphatase (ATPase) and Na(+)-K(+)-2Cl- cotransport partially accounted for vascular smooth muscle cell (VSMC) K+ (Rb+) uptake. In other cells Rb+ is taken up by a K(+)-H(+)-ATPase that is sensitive to NC-1300-B, SCH28080, omeprazole, and N-ethylmaleimide (NEM). This study examines the effects of K(+)-H(+)-ATPase inhibitors on VSMC. Rubidium uptake by primary cultures of canine coronary artery (CCA) VSMC or cultured rat aortic (CRA) VSMC line A7r5 was reduced 19-37% by NC-1300-B, SCH28080, or omeprazole. N-ethylmaleimide reduced CCA VSMC K+ content from 1.55 +/- 0.02 to 1.24 +/- 0.06 mu eq/mg protein. The NC-1300-B-sensitive portion of CRA VSMC Rb+ uptake was not blocked by ouabain (0.1 mM) or bumetanide (0.1 mM), but was reduced by alkalinization with 7.5 mM NH4Cl, and increased by acidification with 7.5 mM Na-acetate. Intracellular pH (pHi) of CRA VSMC was reduced 0.14 +/- 0.03 U by NC-1300-B and 0.22 +/- 0.03 U by NEM. pHi of CCA VSMC was reduced 0.20 +/- 0.03 U by omeprazole (1 mM) and 0.20 +/- 0.03 U or 0.20 +/- 0.05 U by amiloride in the absence or presence of omeprazole, respectively. Fluorescence of 2',7'-bis(carboxyethyl)-5-(6')- carboxyethyl)-5-(6')-carboxyfluorescein due to excitation at 500:441 nm in rat aortic strips was reduced by 0.21 +/- 0.02 U by omeprazole and 0.22 +/- 0.03 U by K+ removal and increased by 0.21 +/- 0.06 U by K+ repletion. We conclude that VSMC possess a previously unknown Rb+ uptake mechanism. This newly discovered mechanism helps to maintain K+ gradient and pHi by extruding H+ in exchange for K+, and is presumably a K(+)-H(+)-ATPase similar to those described in other tissues." @default.
- W1859392330 created "2016-06-24" @default.
- W1859392330 creator A5008924290 @default.
- W1859392330 creator A5035257319 @default.
- W1859392330 date "1992-06-01" @default.
- W1859392330 modified "2023-09-25" @default.
- W1859392330 title "Evidence of a K(+)-H(+)-ATPase in vascular smooth muscle cells" @default.
- W1859392330 doi "https://doi.org/10.1152/ajpheart.1992.262.6.h1955" @default.
- W1859392330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1320341" @default.
- W1859392330 hasPublicationYear "1992" @default.
- W1859392330 type Work @default.
- W1859392330 sameAs 1859392330 @default.
- W1859392330 citedByCount "9" @default.
- W1859392330 countsByYear W18593923302014 @default.
- W1859392330 countsByYear W18593923302016 @default.
- W1859392330 crossrefType "journal-article" @default.
- W1859392330 hasAuthorship W1859392330A5008924290 @default.
- W1859392330 hasAuthorship W1859392330A5035257319 @default.
- W1859392330 hasConcept C126322002 @default.
- W1859392330 hasConcept C134018914 @default.
- W1859392330 hasConcept C178790620 @default.
- W1859392330 hasConcept C181199279 @default.
- W1859392330 hasConcept C185592680 @default.
- W1859392330 hasConcept C188053792 @default.
- W1859392330 hasConcept C23265538 @default.
- W1859392330 hasConcept C2778212192 @default.
- W1859392330 hasConcept C2779133857 @default.
- W1859392330 hasConcept C2779395532 @default.
- W1859392330 hasConcept C2779608910 @default.
- W1859392330 hasConcept C2992686903 @default.
- W1859392330 hasConcept C41625074 @default.
- W1859392330 hasConcept C537181965 @default.
- W1859392330 hasConcept C55493867 @default.
- W1859392330 hasConcept C71924100 @default.
- W1859392330 hasConcept C79879829 @default.
- W1859392330 hasConcept C86803240 @default.
- W1859392330 hasConcept C99975950 @default.
- W1859392330 hasConceptScore W1859392330C126322002 @default.
- W1859392330 hasConceptScore W1859392330C134018914 @default.
- W1859392330 hasConceptScore W1859392330C178790620 @default.
- W1859392330 hasConceptScore W1859392330C181199279 @default.
- W1859392330 hasConceptScore W1859392330C185592680 @default.
- W1859392330 hasConceptScore W1859392330C188053792 @default.
- W1859392330 hasConceptScore W1859392330C23265538 @default.
- W1859392330 hasConceptScore W1859392330C2778212192 @default.
- W1859392330 hasConceptScore W1859392330C2779133857 @default.
- W1859392330 hasConceptScore W1859392330C2779395532 @default.
- W1859392330 hasConceptScore W1859392330C2779608910 @default.
- W1859392330 hasConceptScore W1859392330C2992686903 @default.
- W1859392330 hasConceptScore W1859392330C41625074 @default.
- W1859392330 hasConceptScore W1859392330C537181965 @default.
- W1859392330 hasConceptScore W1859392330C55493867 @default.
- W1859392330 hasConceptScore W1859392330C71924100 @default.
- W1859392330 hasConceptScore W1859392330C79879829 @default.
- W1859392330 hasConceptScore W1859392330C86803240 @default.
- W1859392330 hasConceptScore W1859392330C99975950 @default.
- W1859392330 hasLocation W18593923301 @default.
- W1859392330 hasLocation W18593923302 @default.
- W1859392330 hasOpenAccess W1859392330 @default.
- W1859392330 hasPrimaryLocation W18593923301 @default.
- W1859392330 hasRelatedWork W142758650 @default.
- W1859392330 hasRelatedWork W1513290991 @default.
- W1859392330 hasRelatedWork W1859392330 @default.
- W1859392330 hasRelatedWork W2005234207 @default.
- W1859392330 hasRelatedWork W2017576346 @default.
- W1859392330 hasRelatedWork W2075069486 @default.
- W1859392330 hasRelatedWork W2104592082 @default.
- W1859392330 hasRelatedWork W2110592389 @default.
- W1859392330 hasRelatedWork W2412923117 @default.
- W1859392330 hasRelatedWork W2418470927 @default.
- W1859392330 isParatext "false" @default.
- W1859392330 isRetracted "false" @default.
- W1859392330 magId "1859392330" @default.
- W1859392330 workType "article" @default.