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• W1859798930 abstract "Previous investigators have demonstrated that lipid biosynthesis by animal endometrium is subject to the control of hormones. Chemical analyses and histochemical studies are available, but there are no previous studies of actual lipid biosynthesis by human endometrium. Fragments of human endometrium, obtained by hysterectomy, were incubated for 4 hours in the patient's serum to which acetate-1-C14 was added. Chloroform-methanol was used to terminate biosynthesis and begin extraction of lipids. Serum lipids served as carriers for the tissue lipids through a separation procedure which segregated phospholipids, sterol esters, triglycerides and the remainder of the total lipids. The incorporation of C14 into each class was determined. The preliminary data suggest that lipid biosynthesis by human endometrium varies with time during the menstrual cycle and is characterized by greater incorporation of label into total lipids, phospholipids, and triglycerides during the proliferative phase than during the secretory phase, with no significant change in the incorporation of C14 into sterol esters throughout the cycle. The nonesterified lipids demonstrate greater synthesis during the proliferative phase than during the secretory phase. The major lipid synthesis is of fatty acids with prompt esterification. The proportion of total lipids esterified is greater during the secretory phase than during the proliferative phase. Moreover, the pattern of esterification may shift at the time of ovulation, with a relative increase in sterol esters. This change reflects less variability in the proportional incorporation of label into the sterol esters and the triglycerides than in the incorporation into phospholipids." @default.
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• W1859798930 date "1966-11-01" @default.
• W1859798930 modified "2023-09-23" @default.
• W1859798930 title "Lipid biosynthesis by human endometrium" @default.
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• W1859798930 doi "https://doi.org/10.1016/0002-9378(66)90411-x" @default.
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