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- W185994074 abstract "Over the past decade, microwave-supported acid proteolysis has emerged as a valuable tool in high throughput proteomic analysis. Its major advantage is speed; proteolysis of complex mixtures takes place in less than 30 minutes. Cleavage occurs selectively and reproducibly at aspartic acid residues and produces peptide products whose tandem mass spectra are database searchable. In addition to higher speed and lower cost, advantages over trypsin digestion include reliable production of peptides from proteins with modified arginine and lysine residues and generation of a more limited mixture of peptides that are larger, on average. Disadvantages include hydrolysis of carbohydrate and phosphate modifications. Research-grade microwave devices provide temperature control within ±5°C for microscale reactions." @default.
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- W185994074 date "2013-01-01" @default.
- W185994074 modified "2023-10-14" @default.
- W185994074 title "Asp-Selective Microwave-Supported Acid Proteolysis" @default.
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- W185994074 doi "https://doi.org/10.1016/b978-0-12-394446-7.00014-5" @default.
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