FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1860953353> ?p ?o ?g. }

• W1860953353 endingPage "e0135359" @default.
• W1860953353 startingPage "e0135359" @default.
• W1860953353 abstract "Extravillous trophoblast (EVT) cells invade the endometrium and the maternal spiral arterioles during the first trimester. Mammary Serine Protease Inhibitor (Maspin, SERPINB5) plays a putative role in regulating the invasive activity of cytotrophoblasts. The maspin gene is silenced in various cancers by an epigenetic mechanism that involves aberrant cytosine methylation. We investigated the effect of the methylation status of the maspin promoter on the maspin expression and the aggressiveness of EVT cells.Western blotting was used to detect the maspin protein expression in EVT cells upon hypoxia. The proliferative ability, the apoptosis rate and the migration and invasiveness were measured with Cell Counting Kit-8 assay, Flow Cytometry technology and Transwell methods. Subsequently, we treated cells with recombinant maspin protein. The methylation degree of maspin promoter region upon hypoxia/ decitabine was detected by bisulfite sequencing PCR and methylation-specific PCR. Finally, we explored the effects of decitabine on maspin protein expression and the aggressiveness of EVT cells.Hypoxia effectively increased maspin protein expression in EVT cells and significantly inhibited their aggressiveness. The addition of recombinant maspin protein inhibited this aggressiveness. Decitabine reduced the methylation in the maspin promoter region and effectively increased the maspin protein expression, which significantly weakened the migration and invasiveness of EVT cells.The methylation status of the maspin promoter is an important factor that affects the migration and invasion of EVT cells during early pregnancy. A decrease in the methylation status can inhibit the migration and invasion of EVT cells to affect placentation and can result in the ischemia and hypoxia of placenta." @default.
• W1860953353 created "2016-06-24" @default.
• W1860953353 creator A5008980559 @default.
• W1860953353 creator A5011122371 @default.
• W1860953353 creator A5017941291 @default.
• W1860953353 creator A5026879877 @default.
• W1860953353 creator A5034430614 @default.
• W1860953353 creator A5039433622 @default.
• W1860953353 creator A5043487121 @default.
• W1860953353 creator A5045094860 @default.
• W1860953353 creator A5051147169 @default.
• W1860953353 creator A5088664989 @default.
• W1860953353 date "2015-08-11" @default.
• W1860953353 modified "2023-10-16" @default.
• W1860953353 title "Promoter Hypomethylation of Maspin Inhibits Migration and Invasion of Extravillous Trophoblast Cells during Placentation" @default.
• W1860953353 cites W1502713173 @default.
• W1860953353 cites W1514807480 @default.
• W1860953353 cites W1566096464 @default.
• W1860953353 cites W1898826556 @default.
• W1860953353 cites W1981570640 @default.
• W1860953353 cites W1982742522 @default.
• W1860953353 cites W1991023274 @default.
• W1860953353 cites W1995667741 @default.
• W1860953353 cites W1996638945 @default.
• W1860953353 cites W2007884882 @default.
• W1860953353 cites W2014102496 @default.
• W1860953353 cites W2016441400 @default.
• W1860953353 cites W2019126301 @default.
• W1860953353 cites W2032665278 @default.
• W1860953353 cites W2033871172 @default.
• W1860953353 cites W2035444091 @default.
• W1860953353 cites W2035956718 @default.
• W1860953353 cites W2038469506 @default.
• W1860953353 cites W2046683708 @default.
• W1860953353 cites W2060602916 @default.
• W1860953353 cites W2061267989 @default.
• W1860953353 cites W2066684338 @default.
• W1860953353 cites W2069175442 @default.
• W1860953353 cites W2071428826 @default.
• W1860953353 cites W2071598652 @default.
• W1860953353 cites W2072177711 @default.
• W1860953353 cites W2086730810 @default.
• W1860953353 cites W2087280324 @default.
• W1860953353 cites W2088850572 @default.
• W1860953353 cites W2092655018 @default.
• W1860953353 cites W2097126485 @default.
• W1860953353 cites W2099111635 @default.
• W1860953353 cites W2123043013 @default.
• W1860953353 cites W2124833440 @default.
• W1860953353 cites W2131449144 @default.
• W1860953353 cites W2140676491 @default.
• W1860953353 cites W2154412334 @default.
• W1860953353 cites W2167785685 @default.
• W1860953353 cites W2169753719 @default.
• W1860953353 cites W2178285855 @default.
• W1860953353 cites W9144849 @default.
• W1860953353 doi "https://doi.org/10.1371/journal.pone.0135359" @default.
• W1860953353 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4532475" @default.
• W1860953353 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26263377" @default.
• W1860953353 hasPublicationYear "2015" @default.
• W1860953353 type Work @default.
• W1860953353 sameAs 1860953353 @default.
• W1860953353 citedByCount "12" @default.
• W1860953353 countsByYear W18609533532016 @default.
• W1860953353 countsByYear W18609533532017 @default.
• W1860953353 countsByYear W18609533532019 @default.
• W1860953353 countsByYear W18609533532020 @default.
• W1860953353 countsByYear W18609533532021 @default.
• W1860953353 countsByYear W18609533532022 @default.
• W1860953353 crossrefType "journal-article" @default.
• W1860953353 hasAuthorship W1860953353A5008980559 @default.
• W1860953353 hasAuthorship W1860953353A5011122371 @default.
• W1860953353 hasAuthorship W1860953353A5017941291 @default.
• W1860953353 hasAuthorship W1860953353A5026879877 @default.
• W1860953353 hasAuthorship W1860953353A5034430614 @default.
• W1860953353 hasAuthorship W1860953353A5039433622 @default.
• W1860953353 hasAuthorship W1860953353A5043487121 @default.
• W1860953353 hasAuthorship W1860953353A5045094860 @default.
• W1860953353 hasAuthorship W1860953353A5051147169 @default.
• W1860953353 hasAuthorship W1860953353A5088664989 @default.
• W1860953353 hasBestOaLocation W18609533531 @default.
• W1860953353 hasConcept C104317684 @default.
• W1860953353 hasConcept C121608353 @default.
• W1860953353 hasConcept C150194340 @default.
• W1860953353 hasConcept C153911025 @default.
• W1860953353 hasConcept C172680121 @default.
• W1860953353 hasConcept C190727270 @default.
• W1860953353 hasConcept C2776953305 @default.
• W1860953353 hasConcept C2777495146 @default.
• W1860953353 hasConcept C2778900538 @default.
• W1860953353 hasConcept C2779013556 @default.
• W1860953353 hasConcept C2779234561 @default.
• W1860953353 hasConcept C33288867 @default.
• W1860953353 hasConcept C41091548 @default.
• W1860953353 hasConcept C45167389 @default.
• W1860953353 hasConcept C502942594 @default.
• W1860953353 hasConcept C54355233 @default.
• W1860953353 hasConcept C86803240 @default.

• next