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• W1862420400 endingPage "6338" @default.
• W1862420400 startingPage "6332" @default.
• W1862420400 abstract "DNA fragments previously shown to be required for expression of the CAR1 (arginase) gene in Saccharomyces cerevisiae and to support transcriptional activation of a reporter gene in a heterologous expression vector were shown to bind purified regulatory protein ABF-1. Two ABF-1 sites were identified in the CAR1 upstream region, one to which ABF-1 protein bound with high affinity and a second to which it bound much less avidly. The higher-affinity ABF-1 binding site upstream of CAR1 was an effective competitor of the HMRE, ARS1 B domain, and COR2-GFI binding sequences for protein binding. Point mutations in the CAR1 high-affinity ABF-1 binding site resulted in a 12-fold loss of transcriptional activation of a reporter gene compared with the wild-type CAR1 DNA fragment. These data are consistent with the suggestion that ABF-1 protein is one of the transcription factors involved in expression of the CAR1 gene." @default.
• W1862420400 created "2016-06-24" @default.
• W1862420400 creator A5024200770 @default.
• W1862420400 creator A5047430252 @default.
• W1862420400 date "1991-10-01" @default.
• W1862420400 modified "2023-09-27" @default.
• W1862420400 title "Participation of ABF-1 protein in expression of the Saccharomyces cerevisiae CAR1 gene" @default.
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• W1862420400 doi "https://doi.org/10.1128/jb.173.20.6332-6338.1991" @default.
• W1862420400 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/208964" @default.
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