FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1863103417> ?p ?o ?g. }

• W1863103417 endingPage "6405" @default.
• W1863103417 startingPage "6395" @default.
• W1863103417 abstract "One of the six putative accessory genes of bovine immunodeficiency virus (BIV) is similar to those identified as rev in the human immunodeficiency virus and visna virus genomes. To further analyze the BIV rev gene locus, protein, and function, rev cDNAs were cloned and characterized. BIV rev mRNA is derived from the full-length transcript by multiple splicing events and consists of three exons, including the untranslated leader sequence and two coding exons. BIV rev cDNA was expressed in bacteria and in a mammalian in vitro translation expression system. A 23-kDa Rev protein (p23rev) was immunologically detected in lysates from both systems by using an antiserum made to a synthetic Rev peptide. Recombinant p23rev made in bacteria was purified and used to make a polyvalent antiserum. Antisera to Rev peptide and recombinant p23rev immunoprecipitated p23rev from BIV-infected mammalian cells but not from virions. A mammalian expression vector using the BIV rev cDNA was constructed; p23rev was immunoprecipitated with anti-Rev serum from 32P-labeled lysates of monkey cells transfected with this plasmid, demonstrating that BIV Rev is phosphorylated. Immunofluorescence and immunoelectron microscopy with anti-BIV Rev antisera localized Rev in the nucleus and, particularly, in the nucleoli of BIV-infected cells. In functional studies, the expression of BIV Rev was shown to positively regulate the appearance both of Gag protein, which is translated from the unspliced primary viral transcript, and of singly spliced env mRNA but not that of the multiply spliced tat mRNA. These results demonstrate that BIV Rev activity correlates with the known function of lentivirus Rev proteins." @default.
• W1863103417 created "2016-06-24" @default.
• W1863103417 creator A5047071553 @default.
• W1863103417 creator A5051134735 @default.
• W1863103417 creator A5056182587 @default.
• W1863103417 creator A5066233901 @default.
• W1863103417 creator A5067844076 @default.
• W1863103417 creator A5069268501 @default.
• W1863103417 date "1993-11-01" @default.
• W1863103417 modified "2023-09-23" @default.
• W1863103417 title "Characterization of bovine immunodeficiency virus rev cDNAs and identification and subcellular localization of the Rev protein" @default.
• W1863103417 cites W1485652448 @default.
• W1863103417 cites W1493811887 @default.
• W1863103417 cites W1498175978 @default.
• W1863103417 cites W1531523637 @default.
• W1863103417 cites W1541126279 @default.
• W1863103417 cites W1544851733 @default.
• W1863103417 cites W1547625320 @default.
• W1863103417 cites W1582476922 @default.
• W1863103417 cites W1591944984 @default.
• W1863103417 cites W1607332024 @default.
• W1863103417 cites W1659684293 @default.
• W1863103417 cites W1758506944 @default.
• W1863103417 cites W1793297159 @default.
• W1863103417 cites W1811885229 @default.
• W1863103417 cites W1840089255 @default.
• W1863103417 cites W1843557974 @default.
• W1863103417 cites W1845236136 @default.
• W1863103417 cites W1873199501 @default.
• W1863103417 cites W1886007601 @default.
• W1863103417 cites W1965520579 @default.
• W1863103417 cites W1975993749 @default.
• W1863103417 cites W1977750314 @default.
• W1863103417 cites W1981457569 @default.
• W1863103417 cites W1986894155 @default.
• W1863103417 cites W1989791734 @default.
• W1863103417 cites W1993719557 @default.
• W1863103417 cites W1998093349 @default.
• W1863103417 cites W1999390183 @default.
• W1863103417 cites W1999668682 @default.
• W1863103417 cites W2009310436 @default.
• W1863103417 cites W2013851260 @default.
• W1863103417 cites W2018048765 @default.
• W1863103417 cites W2018289835 @default.
• W1863103417 cites W2019582257 @default.
• W1863103417 cites W2021080732 @default.
• W1863103417 cites W2023250124 @default.
• W1863103417 cites W2030588812 @default.
• W1863103417 cites W2031985285 @default.
• W1863103417 cites W2038352589 @default.
• W1863103417 cites W2039233479 @default.
• W1863103417 cites W2045023644 @default.
• W1863103417 cites W2048060294 @default.
• W1863103417 cites W2053037116 @default.
• W1863103417 cites W2054000570 @default.
• W1863103417 cites W2090454675 @default.
• W1863103417 cites W2101108802 @default.
• W1863103417 cites W2109669557 @default.
• W1863103417 cites W2134812217 @default.
• W1863103417 cites W2138270253 @default.
• W1863103417 cites W2412794328 @default.
• W1863103417 cites W2462637153 @default.
• W1863103417 doi "https://doi.org/10.1128/jvi.67.11.6395-6405.1993" @default.
• W1863103417 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/238074" @default.
• W1863103417 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8411341" @default.
• W1863103417 hasPublicationYear "1993" @default.
• W1863103417 type Work @default.
• W1863103417 sameAs 1863103417 @default.
• W1863103417 citedByCount "31" @default.
• W1863103417 countsByYear W18631034172013 @default.
• W1863103417 countsByYear W18631034172017 @default.
• W1863103417 countsByYear W18631034172018 @default.
• W1863103417 crossrefType "journal-article" @default.
• W1863103417 hasAuthorship W1863103417A5047071553 @default.
• W1863103417 hasAuthorship W1863103417A5051134735 @default.
• W1863103417 hasAuthorship W1863103417A5056182587 @default.
• W1863103417 hasAuthorship W1863103417A5066233901 @default.
• W1863103417 hasAuthorship W1863103417A5067844076 @default.
• W1863103417 hasAuthorship W1863103417A5069268501 @default.
• W1863103417 hasBestOaLocation W18631034171 @default.
• W1863103417 hasConcept C104317684 @default.
• W1863103417 hasConcept C153911025 @default.
• W1863103417 hasConcept C159047783 @default.
• W1863103417 hasConcept C159654299 @default.
• W1863103417 hasConcept C187882448 @default.
• W1863103417 hasConcept C2522874641 @default.
• W1863103417 hasConcept C54355233 @default.
• W1863103417 hasConcept C54458228 @default.
• W1863103417 hasConcept C67705224 @default.
• W1863103417 hasConcept C86803240 @default.
• W1863103417 hasConcept C91523082 @default.
• W1863103417 hasConceptScore W1863103417C104317684 @default.
• W1863103417 hasConceptScore W1863103417C153911025 @default.
• W1863103417 hasConceptScore W1863103417C159047783 @default.
• W1863103417 hasConceptScore W1863103417C159654299 @default.
• W1863103417 hasConceptScore W1863103417C187882448 @default.
• W1863103417 hasConceptScore W1863103417C2522874641 @default.
• W1863103417 hasConceptScore W1863103417C54355233 @default.

• next