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- W1865487079 abstract "A coagulant protein called trombin like enzyme, was purified from the venom of the Bothrops pictus peruvian snake, using two chromatographic steps: Sephadex G-100 SF and CM Sephadex C-50, equilibrated with 0,05 M ammonium acetate buffer pH 6,0. The enzyme was purified 18 folds with 40,74% of yield and the PAGE-SDS analysis showed an unit protein band with 45,4 kDa and of 54,4 kDa under no reducing and reducing s. Thus it is a single polypeptide chain with at least a S-S bond. The enzyme was capable to clot bovine fibrinogen and attack BApNA chromogenic substrate; being an inhibitor agent as well as PMSF serinoprotease inhibitor. It had a 8,0 as optimum pH on BApNA and it was stable until 50 °C. 2+ Mn ion (10 mM) was a activator instead." @default.
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- W1865487079 date "2011-01-07" @default.
- W1865487079 modified "2023-09-24" @default.
- W1865487079 title "Purificación y caracterización de un nuevo principio coagulante del veneno de la serpiente peruana Bothrops pictus" @default.
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