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- W18671211 abstract "Recent advancements in recombinant technology have proved to be a promising andeffective approach for more sustainable large scale productions of many therapeuticproteins. Nevertheless, since this approach involves expression of protein in a nonnativehost microorganism, the overall production processes are not quite that straightforwardas several common challenges, such as protein degradation, especially duringcell disruption stage. As the process has been subjected to both protein and hostspecific,a systematic process “conditioning” for maximal production of recombinantprotein is therefore required. In this study, a simple approach to determine optimalconditions for cell disruption using high-pressure homogenizer method to isolaterecombinant bromelian from E. coli BL21-AI is reported. One-Factor-At-A-Time(OFAT) approach was initially used to determine optimal test range for each parameter,namely, pressure (10-50 MPa), flow rate (10-20 psi) and number of passes (1-3 cycles).Next, by response surface methodology (RSM) a three factors face-centered centralcomposite design (FCCD) was applied to obtain optimal process conditions Then theprediction model was validated. Under the optimal conditions, the specific enzymeactivity of the recombinant bromelain was found to be functionally and reproduciblyacceptable at 0.037 U/mg." @default.
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- W18671211 date "2012-01-01" @default.
- W18671211 modified "2023-09-27" @default.
- W18671211 title "Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial productionCOMMERCIAL PRODUCTION" @default.
- W18671211 hasPublicationYear "2012" @default.
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