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• W1867284802 endingPage "e1005162" @default.
• W1867284802 startingPage "e1005162" @default.
• W1867284802 abstract "The ability of pathogens to cause disease depends on their aptitude to escape the immune system. Type IV pili are extracellular filamentous virulence factors composed of pilin monomers and frequently expressed by bacterial pathogens. As such they are major targets for the host immune system. In the human pathogen Neisseria meningitidis, strains expressing class I pilins contain a genetic recombination system that promotes variation of the pilin sequence and is thought to aid immune escape. However, numerous hypervirulent clinical isolates express class II pilins that lack this property. This raises the question of how they evade immunity targeting type IV pili. As glycosylation is a possible source of antigenic variation it was investigated using top-down mass spectrometry to provide the highest molecular precision on the modified proteins. Unlike class I pilins that carry a single glycan, we found that class II pilins display up to 5 glycosylation sites per monomer on the pilus surface. Swapping of pilin class and genetic background shows that the pilin primary structure determines multisite glycosylation while the genetic background determines the nature of the glycans. Absence of glycosylation in class II pilins affects pilus biogenesis or enhances pilus-dependent aggregation in a strain specific fashion highlighting the extensive functional impact of multisite glycosylation. Finally, molecular modeling shows that glycans cover the surface of class II pilins and strongly decrease antibody access to the polypeptide chain. This strongly supports a model where strains expressing class II pilins evade the immune system by changing their sugar structure rather than pilin primary structure. Overall these results show that sequence invariable class II pilins are cloaked in glycans with extensive functional and immunological consequences." @default.
• W1867284802 created "2016-06-24" @default.
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• W1867284802 date "2015-09-14" @default.
• W1867284802 modified "2023-10-16" @default.
• W1867284802 title "Neisseria meningitidis Type IV Pili Composed of Sequence Invariable Pilins Are Masked by Multisite Glycosylation" @default.
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• W1867284802 doi "https://doi.org/10.1371/journal.ppat.1005162" @default.
• W1867284802 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4569582" @default.
• W1867284802 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26367394" @default.
• W1867284802 hasPublicationYear "2015" @default.
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