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- W186760938 abstract "Ion microscopy is a direct imaging mass spectrometry technique which reveals elemental (mass) distribution in relation to tissue morphology. The influence of vitamin D on intestinal Ca transport was examined in parallel experiments by injecting either {sup 47}Ca or {sup 44}Ca into the duodenal lumen of vitamin D-deficient ({minus}D) and vitamin D-replete (+D) chicks for varying periods of time. {sup 47}Ca data provided a quantitative index of tissue retention and absorption, whereas {sup 44}Ca imaging allowed visual localization of Ca specifically in transit from the lumen versus ambient tissue {sup 40}Ca which was also imaged. Luminally-administered Ca rapidly (2.5 minutes) entered the intestinal cells in both the {minus}D and +D chicks, localizing primarily in the area subjacent to the limiting membrane. For the +D chicks, the concentration of {sup 44}Ca in this region dissipated with time, leading to a more homogeneous intracellular distribution as transport proceeded. In contrast, {sup 44}Ca continued to accumulate in the apical cell region in the {minus}D chicks for up to 20 minutes, although a slight inwardly-directed gradient developed. These approaches provide unique information concerning the sequential localization of Ca during intestinal transport and the dynamics of the action of vitamin D thereon." @default.
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- W186760938 date "1990-02-26" @default.
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- W186760938 title "Intestinal calcium transport: An ion microscopic imaging study" @default.
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