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• W1867794489 abstract "This chapter describes the preparation and light-directed activation of caged proteins. The chapter also focuses on methods for perturbing actin-binding protein (ABP) activity in complex molecular environments and for monitoring the state of ABP reactions, using a fluorescent actin conjugate. A major challenge in cell biology is to understand the regulation of cell motility in terms of the multiple protein-mediated reactions that drive this complex process. These mechanism-based studies require knowledge of the intracellular function and regulation of the individual reactions that collectively drive motility. An emerging practice in cell biology is to study specific reactions of a process within the context of the biological system, for example, within a living cell. The protein preparation should be highly purified according to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis and exhibit maximal activity. Protein activity is assayed before the caging reaction. The main limitation of localized photoactivation of caged proteins within a cell is rapid diffusion, which lowers the effective concentration of the protein below the threshold level of activity." @default.
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• W1867794489 date "2003-01-01" @default.
• W1867794489 modified "2023-09-25" @default.
• W1867794489 title "[11] Preparation and light-directed activation of caged proteins" @default.
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• W1867794489 doi "https://doi.org/10.1016/s0076-6879(03)60115-1" @default.
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