FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1870048299> ?p ?o ?g. }

• W1870048299 endingPage "274" @default.
• W1870048299 startingPage "268" @default.
• W1870048299 abstract "The atomic structure of a bacterial aryl acylamidase (EC 3.5.1.13; AAA) is reported and structural features are investigated to better understand the catalytic profile of this enzyme. Structures of AAA were determined in its native form and in complex with the analgesic acetanilide, p-acetaminophenol, at 1.70 Å and 1.73 Å resolutions, respectively. The overall structural fold of AAA was identified as an α/β fold class, exhibiting an open twisted β-sheet core surrounded by α-helices. The asymmetric unit contains one AAA molecule and the monomeric form is functionally active. The core structure enclosing the signature sequence region, including the canonical Ser-cisSer-Lys catalytic triad, is conserved in all members of the Amidase Signature enzyme family. The structure of AAA in a complex with its ligand reveals a unique organization in the substrate-binding pocket. The binding pocket consists of two loops (loop1 and loop2) in the amidase signature sequence and one helix (α10) in the non-amidase signature sequence. We identified two residues (Tyr136 and Thr330) that interact with the ligand via water molecules, and a hydrogen-bonding network that explains the catalytic affinity over various aryl acyl compounds. The optimum activity of AAA at pH > 10 suggests that the reaction mechanism employs Lys84 as the catalytic base to polarize the Ser187 nucleophile in the catalytic triad." @default.
• W1870048299 created "2016-06-24" @default.
• W1870048299 creator A5038079788 @default.
• W1870048299 creator A5051634644 @default.
• W1870048299 creator A5052973870 @default.
• W1870048299 creator A5065493625 @default.
• W1870048299 creator A5077973187 @default.
• W1870048299 creator A5078431879 @default.
• W1870048299 creator A5089238078 @default.
• W1870048299 date "2015-11-01" @default.
• W1870048299 modified "2023-10-17" @default.
• W1870048299 title "Crystal structure analysis of a bacterial aryl acylamidase belonging to the amidase signature enzyme family" @default.
• W1870048299 cites W1507541345 @default.
• W1870048299 cites W1965469766 @default.
• W1870048299 cites W1968212477 @default.
• W1870048299 cites W1986191025 @default.
• W1870048299 cites W1996767019 @default.
• W1870048299 cites W2001641653 @default.
• W1870048299 cites W2030517878 @default.
• W1870048299 cites W2042921052 @default.
• W1870048299 cites W2048254061 @default.
• W1870048299 cites W2051185826 @default.
• W1870048299 cites W2058457787 @default.
• W1870048299 cites W2062751709 @default.
• W1870048299 cites W2090837206 @default.
• W1870048299 cites W2106246537 @default.
• W1870048299 cites W2109184569 @default.
• W1870048299 cites W2137825366 @default.
• W1870048299 cites W2147526198 @default.
• W1870048299 cites W2155553899 @default.
• W1870048299 cites W36449893 @default.
• W1870048299 doi "https://doi.org/10.1016/j.bbrc.2015.09.177" @default.
• W1870048299 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26454172" @default.
• W1870048299 hasPublicationYear "2015" @default.
• W1870048299 type Work @default.
• W1870048299 sameAs 1870048299 @default.
• W1870048299 citedByCount "22" @default.
• W1870048299 countsByYear W18700482992016 @default.
• W1870048299 countsByYear W18700482992017 @default.
• W1870048299 countsByYear W18700482992018 @default.
• W1870048299 countsByYear W18700482992019 @default.
• W1870048299 countsByYear W18700482992020 @default.
• W1870048299 countsByYear W18700482992021 @default.
• W1870048299 countsByYear W18700482992022 @default.
• W1870048299 countsByYear W18700482992023 @default.
• W1870048299 crossrefType "journal-article" @default.
• W1870048299 hasAuthorship W1870048299A5038079788 @default.
• W1870048299 hasAuthorship W1870048299A5051634644 @default.
• W1870048299 hasAuthorship W1870048299A5052973870 @default.
• W1870048299 hasAuthorship W1870048299A5065493625 @default.
• W1870048299 hasAuthorship W1870048299A5077973187 @default.
• W1870048299 hasAuthorship W1870048299A5078431879 @default.
• W1870048299 hasAuthorship W1870048299A5089238078 @default.
• W1870048299 hasConcept C104317684 @default.
• W1870048299 hasConcept C116569031 @default.
• W1870048299 hasConcept C145770059 @default.
• W1870048299 hasConcept C167625842 @default.
• W1870048299 hasConcept C170493617 @default.
• W1870048299 hasConcept C181199279 @default.
• W1870048299 hasConcept C185592680 @default.
• W1870048299 hasConcept C2779485144 @default.
• W1870048299 hasConcept C41183919 @default.
• W1870048299 hasConcept C45484198 @default.
• W1870048299 hasConcept C55493867 @default.
• W1870048299 hasConcept C65588867 @default.
• W1870048299 hasConcept C71240020 @default.
• W1870048299 hasConcept C8010536 @default.
• W1870048299 hasConceptScore W1870048299C104317684 @default.
• W1870048299 hasConceptScore W1870048299C116569031 @default.
• W1870048299 hasConceptScore W1870048299C145770059 @default.
• W1870048299 hasConceptScore W1870048299C167625842 @default.
• W1870048299 hasConceptScore W1870048299C170493617 @default.
• W1870048299 hasConceptScore W1870048299C181199279 @default.
• W1870048299 hasConceptScore W1870048299C185592680 @default.
• W1870048299 hasConceptScore W1870048299C2779485144 @default.
• W1870048299 hasConceptScore W1870048299C41183919 @default.
• W1870048299 hasConceptScore W1870048299C45484198 @default.
• W1870048299 hasConceptScore W1870048299C55493867 @default.
• W1870048299 hasConceptScore W1870048299C65588867 @default.
• W1870048299 hasConceptScore W1870048299C71240020 @default.
• W1870048299 hasConceptScore W1870048299C8010536 @default.
• W1870048299 hasFunder F4320321373 @default.
• W1870048299 hasIssue "2" @default.
• W1870048299 hasLocation W18700482991 @default.
• W1870048299 hasLocation W18700482992 @default.
• W1870048299 hasOpenAccess W1870048299 @default.
• W1870048299 hasPrimaryLocation W18700482991 @default.
• W1870048299 hasRelatedWork W1833411842 @default.
• W1870048299 hasRelatedWork W1979983680 @default.
• W1870048299 hasRelatedWork W2006627653 @default.
• W1870048299 hasRelatedWork W2093753737 @default.
• W1870048299 hasRelatedWork W2125039052 @default.
• W1870048299 hasRelatedWork W2143028632 @default.
• W1870048299 hasRelatedWork W2163723468 @default.
• W1870048299 hasRelatedWork W2759274657 @default.
• W1870048299 hasRelatedWork W2796248822 @default.
• W1870048299 hasRelatedWork W2884932018 @default.
• W1870048299 hasVolume "467" @default.

• next