Matches in SemOpenAlex for { <https://semopenalex.org/work/W1870052210> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1870052210 abstract "Transmission electron microscopy (TEM) is a valuable tool for elucidating cilium ultrastructure and the effects of ciliary and ciliopathy gene mutations on cilium integrity. We perform TEM on amphid channel sensory cilia in C. elegans, enabling us to visualise cilium compartments, namely the axoneme, the transition zone (TZ) and the transition fiber/basal body region. In worms, cilia extend from the dendritic tips of 60 sensory neurons. Here we show how TEM can be used to identify ultrastructural defects observed at the TZ of a number of ciliopathy disease gene mutants. In WT worms, the ~1 micron TZ is the most proximal part of the axoneme that extends from the far distal dendrite membrane, and is defined by a drawing together of the 9 doublet microtubules (MT) and Y-links that connect the doublet MTs with the ciliary membrane. In worms with mutations in Meckel-Gruber Syndrome (MKS) and Nephronophthisis (NPHP) gene homologues, we have observed a range of TZ abnormalities. These include TZs with Y-link defects (missing arms, reduced numbers) and TZs that fail to dock at the tip of the dendritic compartment. In most instances, TZ defects are observed only in worms where both MKS and NPHP functional modules are genetically disrupted and not in worms where only one module is abrogated. Together, our observations indicate redundant roles for multiple TZ-associated ciliopathy proteins in establishing physical connections between the TZ and the ciliary membrane." @default.
- W1870052210 created "2016-06-24" @default.
- W1870052210 creator A5004839255 @default.
- W1870052210 creator A5031592364 @default.
- W1870052210 creator A5038131406 @default.
- W1870052210 creator A5083318346 @default.
- W1870052210 date "2012-11-01" @default.
- W1870052210 modified "2023-09-24" @default.
- W1870052210 title "A picture tells a thousand words: transmission electron microscopy of the ciliary transition zone in C. elegans" @default.
- W1870052210 doi "https://doi.org/10.1186/2046-2530-1-s1-p18" @default.
- W1870052210 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3555792" @default.
- W1870052210 hasPublicationYear "2012" @default.
- W1870052210 type Work @default.
- W1870052210 sameAs 1870052210 @default.
- W1870052210 citedByCount "0" @default.
- W1870052210 crossrefType "journal-article" @default.
- W1870052210 hasAuthorship W1870052210A5004839255 @default.
- W1870052210 hasAuthorship W1870052210A5031592364 @default.
- W1870052210 hasAuthorship W1870052210A5038131406 @default.
- W1870052210 hasAuthorship W1870052210A5083318346 @default.
- W1870052210 hasBestOaLocation W18700522101 @default.
- W1870052210 hasConcept C100094513 @default.
- W1870052210 hasConcept C104317684 @default.
- W1870052210 hasConcept C105702510 @default.
- W1870052210 hasConcept C115811362 @default.
- W1870052210 hasConcept C127716648 @default.
- W1870052210 hasConcept C131151476 @default.
- W1870052210 hasConcept C143065580 @default.
- W1870052210 hasConcept C195267511 @default.
- W1870052210 hasConcept C24870808 @default.
- W1870052210 hasConcept C2778215940 @default.
- W1870052210 hasConcept C2780561973 @default.
- W1870052210 hasConcept C2910722709 @default.
- W1870052210 hasConcept C37590093 @default.
- W1870052210 hasConcept C54355233 @default.
- W1870052210 hasConcept C86803240 @default.
- W1870052210 hasConcept C87555872 @default.
- W1870052210 hasConcept C95444343 @default.
- W1870052210 hasConceptScore W1870052210C100094513 @default.
- W1870052210 hasConceptScore W1870052210C104317684 @default.
- W1870052210 hasConceptScore W1870052210C105702510 @default.
- W1870052210 hasConceptScore W1870052210C115811362 @default.
- W1870052210 hasConceptScore W1870052210C127716648 @default.
- W1870052210 hasConceptScore W1870052210C131151476 @default.
- W1870052210 hasConceptScore W1870052210C143065580 @default.
- W1870052210 hasConceptScore W1870052210C195267511 @default.
- W1870052210 hasConceptScore W1870052210C24870808 @default.
- W1870052210 hasConceptScore W1870052210C2778215940 @default.
- W1870052210 hasConceptScore W1870052210C2780561973 @default.
- W1870052210 hasConceptScore W1870052210C2910722709 @default.
- W1870052210 hasConceptScore W1870052210C37590093 @default.
- W1870052210 hasConceptScore W1870052210C54355233 @default.
- W1870052210 hasConceptScore W1870052210C86803240 @default.
- W1870052210 hasConceptScore W1870052210C87555872 @default.
- W1870052210 hasConceptScore W1870052210C95444343 @default.
- W1870052210 hasLocation W18700522101 @default.
- W1870052210 hasLocation W18700522102 @default.
- W1870052210 hasLocation W18700522103 @default.
- W1870052210 hasOpenAccess W1870052210 @default.
- W1870052210 hasPrimaryLocation W18700522101 @default.
- W1870052210 hasRelatedWork W2009004971 @default.
- W1870052210 hasRelatedWork W2011983184 @default.
- W1870052210 hasRelatedWork W2018716885 @default.
- W1870052210 hasRelatedWork W2053893960 @default.
- W1870052210 hasRelatedWork W2060941388 @default.
- W1870052210 hasRelatedWork W2061448982 @default.
- W1870052210 hasRelatedWork W2094911558 @default.
- W1870052210 hasRelatedWork W2099247534 @default.
- W1870052210 hasRelatedWork W2130086914 @default.
- W1870052210 hasRelatedWork W2140100347 @default.
- W1870052210 hasRelatedWork W2161870393 @default.
- W1870052210 hasRelatedWork W2180541157 @default.
- W1870052210 hasRelatedWork W2509850786 @default.
- W1870052210 hasRelatedWork W2515973749 @default.
- W1870052210 hasRelatedWork W2538387512 @default.
- W1870052210 hasRelatedWork W2615491118 @default.
- W1870052210 hasRelatedWork W2808100256 @default.
- W1870052210 hasRelatedWork W2952261609 @default.
- W1870052210 hasRelatedWork W2995396845 @default.
- W1870052210 hasRelatedWork W617284070 @default.
- W1870052210 isParatext "false" @default.
- W1870052210 isRetracted "false" @default.
- W1870052210 magId "1870052210" @default.
- W1870052210 workType "article" @default.