FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W187011570> ?p ?o ?g. }

• W187011570 endingPage "699" @default.
• W187011570 startingPage "699" @default.
• W187011570 abstract "To explore the methods of hepatocytes culture in a collagen gel mixture or between double layers of collagen sandwich configuration and to examine the functional and cytomorphological characteristics of cultured hepatocytes.A two-step collagenase perfusion technique was used to isolate the hepatocytes from Wistar rats or newborn Chinese experimental piglets. The isolated hepatocytes were cultured in a collagen gel mixture or between double layers of collagen sandwich configuration respectively. The former was that rat hepatocytes were mixed with type I rat tail collagen solution till gelled, and the medium was added onto the gel. The latter was that swine hepatocytes were seeded on a plate precoated with collagen gel for 24 h, then another layer of collagen gel was overlaid, resulting in a sandwich configuration. The cytomorphological characteristics, albumin secretion, and LDH-release of the hepatocytes cultured in these two models were examined.Freshly isolated rat hepatocytes were successfully mixed and fixed in collagen gel, and cultured in the gel condition. During the culture period, the urea synthesized and secreted by rat hepatocytes was detected throughout the period. Likewise, newborn experimental piglet hepatocytes were successfully fixed between the double layers of collagen gel, forming a sandwich configuration. Within a week of culture, the albumin secreted by swine hepatocytes was detected by SDS/PAGE analysis. The typical cytomorphological characteristics of the hepatocytes cultured by the above two culture models were found under a phase-contrast microscope. There was little LDH-release during the culture period.Both collagen gel mixture and double layers of collagen sandwich configuration can provide cultural conditions much closer to in vivo environment, and are helpful for maintaining specific hepatic functions and cytomorphological characteristics. A collagen gel mixture culture may be more eligible for the study of bioartificial livers." @default.
• W187011570 created "2016-06-24" @default.
• W187011570 creator A5020487588 @default.
• W187011570 creator A5039895229 @default.
• W187011570 creator A5041364635 @default.
• W187011570 creator A5041748603 @default.
• W187011570 creator A5045284378 @default.
• W187011570 date "2004-01-01" @default.
• W187011570 modified "2023-10-13" @default.
• W187011570 title "Primary hepatocyte culture in collagen gel mixture and collagen sandwich" @default.
• W187011570 cites W155464466 @default.
• W187011570 cites W191325192 @default.
• W187011570 cites W193402779 @default.
• W187011570 cites W1970878708 @default.
• W187011570 cites W1973366984 @default.
• W187011570 cites W1996589982 @default.
• W187011570 cites W2025377144 @default.
• W187011570 cites W2027413685 @default.
• W187011570 cites W2062530492 @default.
• W187011570 cites W2080034461 @default.
• W187011570 cites W2094519708 @default.
• W187011570 cites W2132006762 @default.
• W187011570 cites W2168479803 @default.
• W187011570 cites W2177164585 @default.
• W187011570 cites W2221265469 @default.
• W187011570 cites W2263252693 @default.
• W187011570 cites W2294939829 @default.
• W187011570 cites W2295721027 @default.
• W187011570 cites W2304393103 @default.
• W187011570 cites W2334558331 @default.
• W187011570 cites W2392307657 @default.
• W187011570 cites W2403031218 @default.
• W187011570 cites W2403903970 @default.
• W187011570 cites W2413851796 @default.
• W187011570 cites W2414038285 @default.
• W187011570 cites W2415917762 @default.
• W187011570 cites W2416505398 @default.
• W187011570 cites W2474558024 @default.
• W187011570 doi "https://doi.org/10.3748/wjg.v10.i5.699" @default.
• W187011570 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4716912" @default.
• W187011570 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14991941" @default.
• W187011570 hasPublicationYear "2004" @default.
• W187011570 type Work @default.
• W187011570 sameAs 187011570 @default.
• W187011570 citedByCount "41" @default.
• W187011570 countsByYear W1870115702012 @default.
• W187011570 countsByYear W1870115702013 @default.
• W187011570 countsByYear W1870115702014 @default.
• W187011570 countsByYear W1870115702015 @default.
• W187011570 countsByYear W1870115702016 @default.
• W187011570 countsByYear W1870115702017 @default.
• W187011570 countsByYear W1870115702018 @default.
• W187011570 countsByYear W1870115702019 @default.
• W187011570 countsByYear W1870115702020 @default.
• W187011570 countsByYear W1870115702021 @default.
• W187011570 countsByYear W1870115702022 @default.
• W187011570 countsByYear W1870115702023 @default.
• W187011570 crossrefType "journal-article" @default.
• W187011570 hasAuthorship W187011570A5020487588 @default.
• W187011570 hasAuthorship W187011570A5039895229 @default.
• W187011570 hasAuthorship W187011570A5041364635 @default.
• W187011570 hasAuthorship W187011570A5041748603 @default.
• W187011570 hasAuthorship W187011570A5045284378 @default.
• W187011570 hasBestOaLocation W1870115701 @default.
• W187011570 hasConcept C134018914 @default.
• W187011570 hasConcept C153911025 @default.
• W187011570 hasConcept C176891718 @default.
• W187011570 hasConcept C181199279 @default.
• W187011570 hasConcept C185592680 @default.
• W187011570 hasConcept C202751555 @default.
• W187011570 hasConcept C2776125364 @default.
• W187011570 hasConcept C2776200302 @default.
• W187011570 hasConcept C2778394429 @default.
• W187011570 hasConcept C2780644872 @default.
• W187011570 hasConcept C55493867 @default.
• W187011570 hasConcept C86803240 @default.
• W187011570 hasConceptScore W187011570C134018914 @default.
• W187011570 hasConceptScore W187011570C153911025 @default.
• W187011570 hasConceptScore W187011570C176891718 @default.
• W187011570 hasConceptScore W187011570C181199279 @default.
• W187011570 hasConceptScore W187011570C185592680 @default.
• W187011570 hasConceptScore W187011570C202751555 @default.
• W187011570 hasConceptScore W187011570C2776125364 @default.
• W187011570 hasConceptScore W187011570C2776200302 @default.
• W187011570 hasConceptScore W187011570C2778394429 @default.
• W187011570 hasConceptScore W187011570C2780644872 @default.
• W187011570 hasConceptScore W187011570C55493867 @default.
• W187011570 hasConceptScore W187011570C86803240 @default.
• W187011570 hasIssue "5" @default.
• W187011570 hasLocation W1870115701 @default.
• W187011570 hasLocation W1870115702 @default.
• W187011570 hasLocation W1870115703 @default.
• W187011570 hasLocation W1870115704 @default.
• W187011570 hasOpenAccess W187011570 @default.
• W187011570 hasPrimaryLocation W1870115701 @default.
• W187011570 hasRelatedWork W1966328299 @default.
• W187011570 hasRelatedWork W2032977619 @default.
• W187011570 hasRelatedWork W2042698048 @default.

• next