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• W1870170011 abstract "Physiological (organismal), biochemical, and molecular biological contributions to the knowledge of the osmoregulatory plasticity of palaemonid freshwater shrimps has provided a fairly complete model of transporter localization in their branchial epithelium. Direct immunological demonstration of the main enzymes in the gill epithelia of adult palaemonids is, however, still incipient. The diadromous freshwater shrimp Macrobrachium acanthurus was exposed to increased salinity (25‰ for 24 hr), and its responses at the systemic level were evaluated through the assays of hemolymph osmolality and muscle hydration, and at cellular and subcellular levels through the activity and localization of the V-H(+) -ATPase, the Na(+) /K(+) -ATPase, and the carbonic anhydrase. Results showed an increase in hemolymph osmolality (629 ± 5.3 mOsm/kg H2 O) and a decrease in muscle hydration (73.8 ± 0.5%), comparing values after 24 hr in 25‰ with control shrimps in freshwater (respectively 409.5 ± 15.8 mOsm/kg H2 O and 77.5 ± 0.4%). V-H(+) -ATPase was localized in pillar cells, whereas Na(+) /K(+) -ATPase in the septal cells. The main novelty of this study was that carbonic anhydrase was localized in the whole branchial tissue, in pillar and septal cells. Exposure to high salinity for 24 hr led to no detectable changes in their localization or in vitro activity. Immunolocalization data corroborated the literature and current models of palaemonid gill ion transport. The absence of changes reinforces the need for the constant expression of these enzymes to account for the euryhalinity of these shrimps." @default.
• W1870170011 created "2016-06-24" @default.
• W1870170011 creator A5001238269 @default.
• W1870170011 creator A5068252935 @default.
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• W1870170011 date "2015-06-02" @default.
• W1870170011 modified "2023-10-05" @default.
• W1870170011 title "Immunocytochemical localization of V-H⁺-ATPase, Na⁺/K⁺-ATPase, and carbonic anhydrase in gill lamellae of adult freshwater euryhaline shrimp<i>Macrobrachium acanthurus</i>(Decapoda, Palaemonidae)" @default.
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• W1870170011 doi "https://doi.org/10.1002/jez.1934" @default.
• W1870170011 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26036663" @default.
• W1870170011 hasPublicationYear "2015" @default.
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