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- W1870760732 abstract "Influence of culture medium on in vitro seed germination and multiplication rate of melissa officinalis L. The present work was carried out aiming to evaluate in vitro germination of Melissa officinalis seeds and multiplication rate. Different sucrose (3% and 1.5%) and MS medium salt concentration (MS, MS/2 and MS/4) were tested for seed germination. For evaluation of multiplication rate, M. officinalis plants were cultured in MS medium (whole concentration) supplemented or not with 4.44 µmol/L BAP. The experiments were carried out in completely randomized design, being the first (germination) with 6 repetitions with 5 tubes and the second (multiplication rate) with 11 repetitions and each one with 4 tubes. In the first experiment, GVI (germination velocity index), seed germination percentage and shoot length were evaluated, whereas in the second experiment shoot number, nodal number, shoot length and root number were evaluated. MS/4 medium led to higher germination velocity index and germination percentage, but greater shoot length was obtained in MS medium. In the second experiment, shoots of M. officinalis cultivated in MS medium containing 4.44 µmol/L of BAP presented larger shoot number, but with a small number of nodes and shoot length, as well as without root formation. Higher multiplication rates were achieved in MS medium." @default.
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- W1870760732 date "2008-01-01" @default.
- W1870760732 modified "2023-09-23" @default.
- W1870760732 title "Influência do meio de cultura na germinação de sementes in vitro e taxa de multiplicação de Melissa officinalis L." @default.
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