Matches in SemOpenAlex for { <https://semopenalex.org/work/W1872826612> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1872826612 endingPage "986" @default.
- W1872826612 startingPage "978" @default.
- W1872826612 abstract "Abstract Several grapevine leafroll‐associated viruses ( GLR aVs) have been found frequently in grapevines behaving GLD . Among them, GLR aV‐2 is the only one belonging to Closterovirus , and mainly induces leafroll symptoms and graft incompatibility. In this study, new degenerate primer pairs designed against the HSP 70 gene were applied in polymerase chain reaction ( PCR ) and nested PCR ( nPCR ) to detect GLR aV‐2 in 132 samples collected from 14 provinces and regions of China. Of the samples, 51.5% were infected with GLR aV‐2, and most did not exhibit GLD symptoms. Some popular grape cultivars had a high incidence of GLR aV‐2 infection, such as Cabernet Sauvignon (92.3%), Chardonnay (80%), Red Globe (75%) and Italian Riesling (73.7%). ‘Beta’ rootstocks, previously identified as negative samples, were also found to be highly infected with GLR aV‐2 (50%). GLR aV‐2 isolates obtained in this study showed identities ranging from 68.9% to 100% and 76.47% to 100.0% at the nucleotide and amino acid levels, respectively. Phylogenetic analysis based on the HSP 70 gene showed that all GLR aV‐2 isolates in China belong to three of five reported phylogenetic groups. Different variants belonging to the PN and RG groups were present in a single isolate. The results showed that the new degenerate primer pairs could detect more GLR aV‐2 isolates than the previously reported primers. This is the first detailed report on the prevalence and gene diversity of GLR aV‐2 in China and also provides an nPCR method to improve the sensitivity of PCR as an alternative method when no real‐time PCR device is available." @default.
- W1872826612 created "2016-06-24" @default.
- W1872826612 creator A5004768789 @default.
- W1872826612 creator A5048449695 @default.
- W1872826612 creator A5058609694 @default.
- W1872826612 creator A5060792525 @default.
- W1872826612 creator A5065141144 @default.
- W1872826612 creator A5080848884 @default.
- W1872826612 date "2015-05-15" @default.
- W1872826612 modified "2023-10-03" @default.
- W1872826612 title "Detection and Sequence Analysis of <i>Grapevine Leafroll-Associated Virus 2</i> Isolates from China" @default.
- W1872826612 cites W1813285591 @default.
- W1872826612 cites W1961077942 @default.
- W1872826612 cites W1966133946 @default.
- W1872826612 cites W1970312482 @default.
- W1872826612 cites W1989266970 @default.
- W1872826612 cites W1992665051 @default.
- W1872826612 cites W2003511339 @default.
- W1872826612 cites W2008959813 @default.
- W1872826612 cites W2027519405 @default.
- W1872826612 cites W2044374300 @default.
- W1872826612 cites W2058309164 @default.
- W1872826612 cites W2060518413 @default.
- W1872826612 cites W2071511674 @default.
- W1872826612 cites W2088312064 @default.
- W1872826612 cites W2090839441 @default.
- W1872826612 cites W2101057672 @default.
- W1872826612 cites W2105275554 @default.
- W1872826612 cites W2109529934 @default.
- W1872826612 cites W2130817816 @default.
- W1872826612 cites W2151089413 @default.
- W1872826612 cites W2152207030 @default.
- W1872826612 doi "https://doi.org/10.1111/jph.12403" @default.
- W1872826612 hasPublicationYear "2015" @default.
- W1872826612 type Work @default.
- W1872826612 sameAs 1872826612 @default.
- W1872826612 citedByCount "8" @default.
- W1872826612 countsByYear W18728266122017 @default.
- W1872826612 countsByYear W18728266122019 @default.
- W1872826612 countsByYear W18728266122020 @default.
- W1872826612 countsByYear W18728266122022 @default.
- W1872826612 crossrefType "journal-article" @default.
- W1872826612 hasAuthorship W1872826612A5004768789 @default.
- W1872826612 hasAuthorship W1872826612A5048449695 @default.
- W1872826612 hasAuthorship W1872826612A5058609694 @default.
- W1872826612 hasAuthorship W1872826612A5060792525 @default.
- W1872826612 hasAuthorship W1872826612A5065141144 @default.
- W1872826612 hasAuthorship W1872826612A5080848884 @default.
- W1872826612 hasConcept C104317684 @default.
- W1872826612 hasConcept C109110057 @default.
- W1872826612 hasConcept C159047783 @default.
- W1872826612 hasConcept C178790620 @default.
- W1872826612 hasConcept C185592680 @default.
- W1872826612 hasConcept C193252679 @default.
- W1872826612 hasConcept C2522874641 @default.
- W1872826612 hasConcept C2777563447 @default.
- W1872826612 hasConcept C2780287141 @default.
- W1872826612 hasConcept C49105822 @default.
- W1872826612 hasConcept C54355233 @default.
- W1872826612 hasConcept C86803240 @default.
- W1872826612 hasConceptScore W1872826612C104317684 @default.
- W1872826612 hasConceptScore W1872826612C109110057 @default.
- W1872826612 hasConceptScore W1872826612C159047783 @default.
- W1872826612 hasConceptScore W1872826612C178790620 @default.
- W1872826612 hasConceptScore W1872826612C185592680 @default.
- W1872826612 hasConceptScore W1872826612C193252679 @default.
- W1872826612 hasConceptScore W1872826612C2522874641 @default.
- W1872826612 hasConceptScore W1872826612C2777563447 @default.
- W1872826612 hasConceptScore W1872826612C2780287141 @default.
- W1872826612 hasConceptScore W1872826612C49105822 @default.
- W1872826612 hasConceptScore W1872826612C54355233 @default.
- W1872826612 hasConceptScore W1872826612C86803240 @default.
- W1872826612 hasIssue "11-12" @default.
- W1872826612 hasLocation W18728266121 @default.
- W1872826612 hasOpenAccess W1872826612 @default.
- W1872826612 hasPrimaryLocation W18728266121 @default.
- W1872826612 hasRelatedWork W2053464542 @default.
- W1872826612 hasRelatedWork W2054377249 @default.
- W1872826612 hasRelatedWork W2054848430 @default.
- W1872826612 hasRelatedWork W2056420846 @default.
- W1872826612 hasRelatedWork W2070179873 @default.
- W1872826612 hasRelatedWork W2086839033 @default.
- W1872826612 hasRelatedWork W2125155197 @default.
- W1872826612 hasRelatedWork W2137190283 @default.
- W1872826612 hasRelatedWork W2160158790 @default.
- W1872826612 hasRelatedWork W2611095818 @default.
- W1872826612 hasVolume "163" @default.
- W1872826612 isParatext "false" @default.
- W1872826612 isRetracted "false" @default.
- W1872826612 magId "1872826612" @default.
- W1872826612 workType "article" @default.